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Structural, functional and immunological studies on a polymeric bacterial protein BJMBR
Baldi,P.C.; Velikovsky,C.A.; Braden,B.C.; Giambartolomei,G.H.; Fossati,C.A.; Goldbaum,F.A..
The characterization of proteins from Brucella spp, the causative agent of brucellosis, has been the subject of intensive research. We have described an 18-kDa cytoplasmic protein of Brucella abortus and shown the potential usefulness of this protein as an antigen for the serologic diagnosis of brucellosis. The amino acid sequence of the protein showed a low but significant homology with that of lumazine synthases. Lumazine is an intermediate product in bacterial riboflavin biosynthesis. The recombinant form of the 18-kDa protein (expressed in E. coli) folds like the native Brucella protein and has lumazine-synthase enzymatic activity. Three-dimensional analysis by X-ray crystallography of the homolog Bacillus subtilis lumazine synthase has revealed that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brucella lumazine synthase; X-ray structure; Immunogenicity; Brucellosis detection; Bacillus subtilis lumazine synthase.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000700003
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