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	Provedor de dados Agrociencia (2) Autor Espinosa-Huerta,Elsa (2) Mora-Avilés,M. Alejandra (2) Quintero-Jiménez,Anareli (2) Acosta-Gallegos,J. Alberto (1) Cabrera-Becerra,K. Virginia (1) Guzmán-Maldonado,H. Salvador (1) Mais... Palavra-chave Bud clusters (2) Gamborg medium (2) Organogenesis (2) Adenine (1) Drought stress tolerance (1) Embryonic axes (1) Fungal resistance (1) Hypocotyls (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2013 (1) 2010 (1) País Mexico (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Stable and efficient Agrobacterium tumefaciens mediated transformation of Phaseolus vulgaris Agrociencia Espinosa-Huerta,Elsa; Quintero-Jiménez,Anareli; Cabrera-Becerra,K. Virginia; Mora-Avilés,M. Alejandra. Common bean breeding requires novel techniques to incorporate genes that may not be available in bean genetic diversity. The objective of the present study was to analyze the main variables involved in Agrobacterium tumefaciens-mediated transformation of common bean towards providing an efficient system to introduce new agronomic traits. Bean cultivars Flor de Mayo Anita and Pinto Saltillo were inoculated with different A. tumefaciens strains, constructs and selection was done using two selection agents (kanamycin ot glufosinateammonium herbicide). Five-day old hypocotyls regenerated organogenic buds (cell clusters) 5 d after inoculation. A randomized complete blocks experimental design was used and the experimental unit was a petri dish with at least 10... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bud clusters; Drought stress tolerance; Fungal resistance; Gamborg medium; Hypocotyls; Organogenesis. Ano: 2013 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952013000400002 An improved method for in vitro regeneration of common bean (Phaseolus vulgaris L.) Agrociencia Quintero-Jiménez,Anareli; Espinosa-Huerta,Elsa; Acosta-Gallegos,J. Alberto; Guzmán-Maldonado,H. Salvador; Mora-Avilés,M. Alejandra. In vitro regeneration of common bean (Phaseolus vulgaris L.) is a requirement for genetic transformation which involves induction and development to the whole plant. Success in regeneration of common bean from tissue organ culture has been achieved to some extent in the last years. However, genotype effects in regeneration response as well as efficiency and reproducibility have been a limiting factor. Embryos, from four different varieties, were excised from sterilized mature seeds and cultured in Murashige and Skoog (MS) or Gamborg (GM) media containing 6-benzylaminopurine (BAP) (10 mg-1) and adenine (A) (0 or 20 mg-1). Efficient regeneration was achieved when inducing formation of differentiation of cells like bud clusters at the internodal segment of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Adenine; Bud clusters; Embryonic axes; Gamborg medium; Organogenesis. Ano: 2010 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952010000100005 Registros recuperados: 2 Primeira ... 1 ... Última

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