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| FARIA,Carla Bertechini; SANTOS,Fabiane Cristina dos; CASTRO,Fausto Fernandes de; SUTIL,Ariadne Ricieli; SERGIO,Luciana Marciano; SILVA,Milena Veronezi; MACHINSKI JUNIOR,Miguel; BARBOSA-TESSMANN,Ione Parra. |
| Abstract Aflatoxins are mutagenic, carcinogenic, and teratogenic mycotoxins. The objective of this work was to study the presence of aflatoxigenic Aspergillus in commercial Bulgur wheat in the city of Maringá, Paraná, Brazil. Thirty samples of commercial Bulgur wheat, acquired in the period of August 2011 to January 2012, were evaluated. The enumeration analysis showed that samples had up to 273.3 CFU of molds and 133.3 CFU of aflatoxigenic Aspergillus per gram of wheat. Forty-two monosporic isolates were obtained and identified as Aspergillus flavus. The isolates were analyzed regarding their aflatoxigenic potential by culture in coconut milk agar; hydroxide vapor exposure; chromatography; and polymerase chain reaction (PCR) targeting genes that code... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aspergillus flavus; Aflatoxins; Bulgur wheat; PCR. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612017000100103 |
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| Faria,Carla Bertechini; Almeida-Ferreira,Giovana Caputo; Gagliardi,Karina Bertechine; Alves,Tatiane Cristina Albuquerque; Tessmann,Dauri José; Machinski Junior,Miguel; Barbosa-Tessmann,Ione Parra. |
| The detection of mycotoxigenic fungi in foodstuff is important because their presence may indicate the possible associated mycotoxin contamination. Fusarium graminearum is a wheat pathogen and a producer of micotoxins. The polymerase chain reaction (PCR) has been employed for the specific identification of F. graminearum. However, this methodology has not been commonly used for detection of F. graminearum in food. Thus, the objective of the present study was to develop a molecular methodology to detect F. graminearum in commercial samples of bulgur wheat. Two methods were tested. In the first method, a sample of this cereal was contaminated with F. graminearum mycelia. The genomic DNA was extracted from this mixture and used in a F. graminearum specific... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bulgur wheat; Fusarium graminearum; PCR; Molecular detection; Food protection. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612012000100030 |
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