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| Registros recuperados: 11 | |
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| Cui,W.; Jin,H.B.; Li,Z.W.. |
| Transforming growth factor-β1 (TGF-β1) plays an important role in the fibrogenic process in the liver. The aim of the present study was to explore the action of TGF-β1 on fibronectin expression in rat hepatic stem-like cells and the underlying mechanisms. The level of fibronectin expression was determined in hepatic stem-like cells (WB cells) before and after TGF-β1 stimulation by RT-PCR and Western blot methods. Using immunogold transmission electron microscopy and the Western blot method, we observed the result of the expression and the distribution of cAMP, phosphorylated Smad3 and Smad7 before and after TGF-β1 treatment. The levels of fibronectin expression in both mRNA and protein increased 4- to 5-fold after TGF-β1 stimulation, reaching an optimum... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: TGF-β; Fibronectin; Fibrogenesis; Hepatic stem cell; CAMP; Smad. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100006 |
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| Xavier-Neto,J.; Pereira,A.C.; Oliveira,E.M.; Miyakawa,A.A.; Junqueira,M.L.; Krieger,J.E.. |
| We characterized the role of potential cAMP-responsive elements (CRE) in basal and in induced angiotensin converting enzyme (ACE) gene promoter activity in order to shed light on the regulation of somatic ACE expression. We identified stimulators and repressors of basal expression between 122 and 288 bp and between 415 and 1303 bp upstream from the transcription start site, respectively, using a rabbit endothelial cell (REC) line. These regions also contained elements associated with the response to 8BrcAMP. When screening for CRE motifs we found pCRE, a proximal sequence between 209 and 222 bp. dCRE, a distal tandem of two CRE-like sequences conserved between rats, mice and humans, was detected between 834 and 846 bp. Gel retardation analysis of nuclear... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Angiotensin converting enzyme; Endothelium; CAMP; Cyclic AMP responsive element. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000002 |
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| Tararthuch,A.L.; Fernandez,R.; Malnic,G.. |
| The interaction between H+ extrusion via H+-ATPase and Cl- conductance was studied in the C11 clone of MDCK cells, akin to the intercalated cells of the collecting duct. Cell pH (pHi) was measured by fluorescence microscopy using the fluorescein-derived probe BCECF-AM. Control recovery rate measured after a 20 mM NH4Cl acid pulse was 0.136 ± 0.008 pH units/min (dpHi/dt) in Na+ Ringer and 0.032 ± 0.003 in the absence of Na+ (0 Na+). With 0 Na+ plus the Cl- channel inhibitor NPPB (10 µM), recovery was reduced to 0.014 ± 0.001 dpHi/dt. 8-Br-cAMP, known to activate CFTR Cl- channels, increased dpHi/dt in 0 Na+ to 0.061 ± 0.009 and also in the presence of 46 nM concanamycin and 50 µM Schering 28080. Since it is thought that the Cl- dependence of H+-ATPase might... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cell pH; CAMP; ATP; H+-ATPase; Valinomycin; Cl-. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000500012 |
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| Ionta,M.; Rosa,M.C.; Almeida,R.B.; Freitas,V.M.; Rezende-Teixeira,P.; Machado-Santelli,G.M.. |
| Hepatocellular carcinoma (HCC) is the third highest cause of cancer death worldwide. In general, the disease is diagnosed at an advanced stage when potentially curative therapies are no longer feasible. For this reason, it is very important to develop new therapeutic approaches. Retinoic acid (RA) is a natural derivative of vitamin A that regulates important biological processes including cell proliferation and differentiation. In vitro studies have shown that RA is effective in inhibiting growth of HCC cells; however, responsiveness to treatment varies among different HCC cell lines. The objective of the present study was to determine if the combined use of RA (0.1 µM) and cAMP (1 mM), an important second messenger, improves the responsiveness of HCC... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cell adhesion; CAMP; Differentiation; Proliferation; Hepatocarcinoma; Retinoic acid. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000800006 |
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| Firmino,Alexandre A.P.; Ulhoa,Cirano J.; Sousa,Marcelo V.; Ferreira Filho,Edivaldo X.; Ricart,Carlos A.O.. |
| The effect of G protein modulators and cyclic AMP (cAMP) on N-acetylglucosaminidase (NAGase) production was investigated during 84 h of growth of a Trichoderma harzianum strain in chitin-containing medium. Caffeine (5 mM), N6--2'-O-dibutyryladenosine 3'5'-cyclic monophosphate sodium salt (dBcAMP) (1 mM) and 3-isobutyl-1-methylxanthine (IBMX) (2 mM) decreased extracellular NAGase activity by 80%, 77% and 37%, respectively. AlCl3/KF (100 µM/10 mM and 200 µM/ 20 mM) decreased the activity by 85% and 95%, respectively. Cholera (10 µ/mL) and pertussis (20 µ/mL) toxins also affected NAGase activity, causing a decrease of approximately 75%. Upon all treatments, protein bands of approximately 73 kDa, 68 kDa and 45 kDa had their signals diminished whilst a 50 kDa... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Trichoderma; N-acetylglucosaminidase; Chitinase; CAMP; G protein. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000200015 |
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| Barbosa,Maria Letícia de Castro; Fumian,Milla Machado; Miranda,Ana Luísa Palhares de; Barreiro,Eliezer J.; Lima,Lídia Moreira. |
| Tumor necrosis factor (TNF) consists of an inflammatory cytokine essential for homeostasis and organism defense. Despite its physiological relevance, both increased biosynthesis and release of TNF lead to the exacerbation of inflammatory and oxidative responses, which are related to the pathogenesis of a host of diseases of an inflammatory, autoimmune and/or infectious nature. In this context, effective therapeutic approaches for the modulation of TNF have been the focus of research efforts. Approximately one million individuals worldwide have been treated with biotechnological inhibitors of this cytokine, the so-called anti-TNF biopharmaceuticals. However, given the high risk of infection and the limitations related to cost and administration routes, new... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tumor necrosis factor; TACE; P38 MAPK; NF-κB; CAMP; Phosphodiesterase; Adenosine receptor. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000300002 |
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| Lou,Dan; Ye,Jifeng; Yang,Lianhua; Wu,Zheng; Zheng,Wei; Zhang,Hui. |
| Icariin, a prenylated flavonol glycoside isolated from Epimedium, has been considered as a potential alternative therapy for osteoporosis. The present study aimed to clarify the detailed molecular mechanisms of action of icariin on osteoblast function, using bone marrow-derived mesenchymal stem cells (BM-MSCs). BM-MSCs were first stimulated by icariin. Then, gene and protein expression of cAMP/PKA/CREB signaling molecules were analyzed by RT-PCR and western blotting (WB), and alkaline phosphatase (ALP) was analyzed in cell lysates by ELISA. MTT assays indicated that icariin did not have significant effects on cell viability up to 1 µM. Icariin showed a dose-dependent effect on the alkaline phosphatase activity of BM-MSCs. WB analysis showed that icariin... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Icariin/molecular mechanisms; BM-MSCs; CAMP; PKA; CREB. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502019000100592 |
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| Elkins, Lynne J.; Meyzen, Christine M.; Callegaro, Sara; Marzoli, Andrea; Bizimis, Michael. |
| The driving processes responsible for producing the Central Atlantic Magmatic Province, the Large Igneous Province associated with end-Triassic rifting of Pangea, remain largely debated. Because their compositions encompass most of the Central Atlantic basalt spectrum, tholeiites from southern Eastern North America are considered pivotal for identifying magma origins. New(176)Hf/Hf-177 measurements for 201 Ma Eastern North American tholeiites dominantly record a local petrogenetic history. Their epsilon(Hf)ratios, corrected to an emplacement age of 201 Ma (-7.85 to +5.86), form a positive but shallowly sloped array slightly deviating from the terrestrial array on a epsilon(Hf)versus epsilon(Nd)diagram. Comparison of(176)Hf/Hf-177 to other isotope ratios... |
| Tipo: Text |
Palavras-chave: Basalt; CAMP; Hafnium; Rift; LIP; Isotope. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00687/79862/82716.pdf |
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| Registros recuperados: 11 | |
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