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| Registros recuperados: 10 | |
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| Munerato,Patrícia; Azevedo,Maria Lúcia; Sucupira,Maria Cecília Araripe; Pardini,Regina; Pinto,Gedson Humberto Novaes; Catroxo,Márcia; Souza,Inara Espinelli; Diaz,Ricardo Sobhie. |
| Entry of human immunodeficiency type 1 virus (HIV-1) into target cells requires both CD4and one of the chemokine receptors. Viruses predominantly use one, or occasionally both, of the major co-receptors CCR5 and CXCR4, although other receptors, including CCR2B and CCR3, function as minor co-receptors. A 32-nucleotide deletion (delta32) within the beta-chemokine receptor 5 gene (CCR5) has been described in subjects who remain uninfected despite extensive exposition to HIV-1. The heterozygous genotype delays disease progression. This allele is common among Caucasians, but has not been found in people of African or Asian ancestry. A more common transition involving a valine to isoleucine switch in transmembrane domain I of CCR2B (64I), with unknown functional... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: CCR5; CCR2B; HIV-1 infection; Polymorphisms. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702003000400002 |
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| Vargas,A.E.; Marrero,A.R.; Salzano,F.M.; Bortolini,M.C.; Chies,J.A.B.. |
| A sample of 103 randomly chosen healthy individuals from Alegrete, RS, Brazil, was tested for the CCR5delta32 allele, which is known to influence susceptibility to HIV-1 infection. The CCR5delta32 allele was identified by PCR amplification using specific primers flanking the region of deletion, followed by electrophoresis on a 3% agarose gel. The data obtained were compared to those reported for other populations and interpreted in terms of Brazilian history. The individuals studied came from a highly admixed population. Most of them were identified as white (N = 59), while blacks and browns (mulattoes) were N = 13 and N = 31, respectively. The observed frequencies, considering the white, black and brown samples (6.8, 3.8, and 6.4%, respectively), suggest... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: CCR5; Chemokine receptors; Brazilian population; Gene flow. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000300002 |
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| Carvalhaes,Fernanda Andreza de Pinho Lott; Cardoso,Greice Lemos; Vallinoto,Antonio Carlos Rosário; Machado,Luiz Fernando; Ishak,Marluisa de Oliveira Guimarães; Ishak,Ricardo; Guerreiro,João Farias. |
| The distribution of genetic polymorphisms of chemokine receptors CCR5-delta32, CCR2-64I and chemokine (SDF1-3A) mutations were studied in 110 Human Immunodeficiency Virus type 1 (HIV-1) seropositive individuals (seropositive group) and 139 seronegative individuals (seronegative group) from the population of the northern Brazilian city of Belém which is the capital of the state of Pará in the Brazilian Amazon. The CCR5-delta32 mutation was found in the two groups at similar frequencies, i.e. 2.2% for the seronegative group and 2.7% for the seropositive group. The frequencies of the SDF1-3A mutation were 21.0% for the seronegative group and 15.4% for the seropositive group, and the CCR2-64I allele was found at frequencies of 12.5% for the seronegative... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: CCR5; CCR2; SDF-1; HIV-1 infection; Allele frequency; Brazilian Amazon. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500004 |
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| Farias,Josileide Duarte de; Santos,Marlene Guimarães; França,Andonai Krauze de; Delani,Daniel; Tada,Mauro Shugiro; Casseb,Almeida Andrade; Simões,Aguinaldo Luiz; Engracia,Vera. |
| Since around 1723, on the occasion of its initial colonization by Europeans, Rondonia has received successive waves of immigrants. This has been further swelled by individuals from northeastern Brazil, who began entering at the beginning of the twentieth century. The ethnic composition varies across the state according to the various sites of settlement of each wave of immigrants. We analyzed the frequency of the CCR5L32 allele of the CCR5 chemokine receptor, which is considered a Caucasian marker, in five sample sets from the population. Four were collected in Porto Velho, the state capital and the site of several waves of migration. Of these, two, from the Hospital de Base were comprised of HB Mothers and HB Newborns presenting allele frequencies of 3.5%... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: CCR5; CCR5Δ32; Rondônia; Genetic polymorphism; Allele frequency. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100003 |
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| Nerys-Junior,Arildo; Costa,Lendel C.; Braga-Dias,Luciene P.; Oliveira,Márcia; Rossi,Átila D.; Cunha,Rodrigo Delvecchio da; Gonçalves,Gabriel S.; Tanuri,Amilcar. |
| Engineered nucleases such as zinc finger nucleases (ZFN) and transcription activator-like effector nucleases (TALEN) are one of the most promising tools for modifying genomes. These site-specific enzymes cause double- strand breaks that allow gene disruption or gene insertion, thereby facilitating genetic manipulation. The major problem associated with this approach is the labor-intensive procedures required to screen and confirm the cellular modification by nucleases. In this work, we produced a TALEN that targets the human CCR5 gene and developed a heteroduplex mobility assay for HEK 293T cells to select positive colonies for sequencing. This approach provides a useful tool for the quick detection and easy assessment of nuclease activity. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: CCR5; Genome editing; Gene knockout; TALEN; HMA. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000100018 |
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| Ibrahim, H. M.; Nishikawa, Y. |
| The current study investigated the impact of the CCR5, TLR2 and TLR11 on production of nitric oxide (NO), IL-6 and IL-12 and growth of T. gondii, high virulent RH strain and avirulent PLK strain. All examined knockout macrophages infected with PLK strain produced significant lower levels of IL-12. On the other hand, TLR2 and TLR11 macrophages infected with RH strain produced significantly reduced levels of IL-12 as compared to wild-type macrophages. TLR2 and TLR11 macrophages infected with PLK strain significantly inhibited the production of IL-6 when compared to wild-type macrophages. Interestingly, significant reduction in the IL-6 and NO production was observed in TLR2 macrophages infected with PLK strain as compared to wild-type macrophages or to the... |
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Palavras-chave: Toxoplasma gondii; CCR5; TLR2; TLR11. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4495 |
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| Registros recuperados: 10 | |
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