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| MEISEL,LEE; FONSECA,BEATRIZ; GONZÁLEZ,SUSANA; BAEZA-YATES,RICARDO; CAMBIAZO,VERONICA; CAMPOS,REINALDO; GONZALEZ,MAURICIO; ORELLANA,ARIEL; RETAMALES,JULIO; SILVA,HERMAN. |
| Prunus persica has been proposed as a genomic model for deciduous trees and the Rosaceae family. Optimized protocols for RNA isolation are necessary to further advance studies in this model species such that functional genomics analyses may be performed. Here we present an optimized protocol to rapidly and efficiently purify high quality total RNA from peach fruits (Prunus persica). Isolating high-quality RNA from fruit tissue is often difficult due to large quantities of polysaccharides and polyphenolic compounds that accumulate in this tissue and co-purify with the RNA. Here we demonstrate that a modified version of the method used to isolate RNA from pine trees and the woody plant Cinnamomun tenuipilum is ideal for isolating high quality RNA from the... |
| Tipo: Journal article |
Palavras-chave: CDNA library; Fruit; Fruit trees; Functional genomics; Peach; Polysaccharides; Prunus persica; RNA isolation; Rosaceae; RT-PCR. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100010 |
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| Cheema,Hafiza Masooma Naseer; Bashir,Aftab; Khatoon,Asia; Iqbal,Nadia; Zafar,Yusuf; Malik,Kauser A. |
| The Calotropis procera seed fibers provide an excellent model system to study the genes involved in fiber elongation, fineness and strength. Expansins constitute one of the important gene families involved in plant cell expansion and other cell wall modification processes. Four homologs of Expansin A gene i.e. CpEXPA1, CpEXPA2, CpEXPA3 and CpEXPA4 were isolated from the cDNA library obtained from fast growing Calotropis procera fibers. These homologs represented typical Expansin A family. Each of them had two conserved domains including GH45 like domain and the putative polysaccharide binding domain. The deduced amino acid sequences of the homologs indicated three conserved motifs: i) eight cysteine residues at N-terminus, ii) four tryptophan residues at... |
| Tipo: Journal article |
Palavras-chave: Calotropis procera; CDNA library; Cotton fibers; EXPANSIN A; Signal peptide; Transcriptome profiling. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600010 |
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| Guarnieri,MC; Melo,ESL; Melo,KMS; Albuquerque-Modesto,JC; Prieto-da-Silva,ARB; Rádis-Baptista,G. |
| The phospholipase A2 superfamily encompasses 15 groups that are classified into: secreted PLA2 (sPLA2); cytosolic PLA2 (cPLA2); Ca2+-independent intracellular PLA2 (iPLA2); platelet-activating factor acetylhydrolase (PAF-AH); and lysosomal PLA2. Currently, approximately 700 PLA2 sequences are known, of which 200 are obtained from the venom gland of Crotalinae snakes. However, thus far, little information is available on cloning, purification and structural characterization of PLA2 from Crotalus durisssus cascavela venom gland. In the present work, we report the molecular cloning of a novel svPLA2 from C. d. cascavella (Cdc), a predominant rattlesnake subspecies in northeastern Brazil. The Cdc svPLA2 cDNA precursor is 689 nucleotides long and encodes a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Molecular toxinology; Crotalus durissus cascavella; Snake venom gland; CDNA library; Acidic PLA2. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000400012 |
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| Fukumoto, Shinya; Sekine, Yukiko; Kimbita, Elikira; Huang, Xiaohong; Xuan, Xuenan; Inoue, Noboru; Yokoyama, Naoaki; Igarashi, Ikuo; Fujisaki, Kozo; Sugimoto, Chihiro; Nagasawa, Hideyuki; Mikami, Takeshi; Suzuki, Hiroshi; 福本, 晋也; 玄, 学南; 井上, 昇; 横山, 直明; 五十嵐, 郁男; 鈴木, 宏志. |
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Palavras-chave: Babesia gibsoni; Babesia canis; CDNA library; ELISA; P30 gene. |
| Ano: 2002 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/624 |
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