| Susceptibility of chicken embryo related cells (CER) to non-classical IBDV was compared with Vero cells for the same purpose. It was used 20 bursal samples collected from clinical cases of IBDV, positive by histopathological evaluation. After three times infection of CER and Vero cells monolayers, at 6, 12, 18, 24, 48, 60 and 72h post-infection (PI), extra-cellular and intracelluar IBDV was measured. The extra-cellular amount of IBDV particles was quantified by DAS-ELISA from 6h PI, and increased for both cells studied until 72h PI. The IBDV re-isolation performed by infecting chicken embryos showed positive results at all PI studied. The intracellular IBDV RNA was shown by RT-PCR, where all tested samples were positive. However, the cytopathic effect... |
