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Bernardes,Ebenézer C.S.; Benko-Iseppon,Ana M.; Vasconcelos,Santelmo; Carvalho,Reginaldo; Brasileiro-Vidal,Ana C.. |
Endive (Cichorium endivia L.) and chicory (C. intybus L.) both have 2n = 18, but until now, there has been no detailed karyomorphological characterization. The present work evaluated five accessions of each species using FISH with rDNA probes and fluorochrome staining with CMA and DAPI. Both species presented distinct banding patterns after fluorochrome staining: while endive had proximal CMA++/DAPI- bands in the short arms of pairs 1, 2 and 3, chicory had proximal CMA-positive bands in chromosomes 1 and 3 and interstitial in the short arm of chromosome 8. Among endive accessions, FISH procedures revealed conserved position and number of 5S and 45S rDNA sites (two and three pairs, respectively), associated with the CMA-positive bands. Notwithstanding,... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Compositae; Cichorieae; CMA/DAPI; RDNA sites; Chromosome rearrangements. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300010 |
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Cordeiro,Joel Maciel Pereira; Felix,Leonardo P.. |
ABSTRACT Although the chromosome number 2n = 28 predominates in most species of the genus Senna, variations are often observed, resulting from either polyploidy (2n = 42, 56, 112) or disploidy (2n = 22, 24, 26) events. To better understand the karyotypic variations in Senna, we examined heterochromatin patterns of 10 species of that genus using chromomycin A3 (CMA) and 4’6-diamidino-2-phenylindole (DAPI) staining, and reviewed information on the chromosome counts of 72 species of that genus. The CMA/DAPI banding patterns were relatively variable among the 10 species, both in terms of the numbers of bands (from two to 26) and their locations on the chromosomes (terminal or proximal regions). Our review indicated that 2n = 28 is the most common ploidy among... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chromosome number; CMA/DAPI; Disploidy; Leguminosae; Polyploidy. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062018000100128 |
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Castro,Juliana P.; Moraes,Ana Paula; Chase,Mark W.; Santos,Angeline M. S.; Batista,Fabiane R. C.; Felix,Leonardo P.. |
ABSTRACT Cactaceae species are karyotypically well-known with x = 11 and chromosome number variation due mainly to polyploidization. However, both assumptions are based on descriptive observations without taking an evolutionary framework of Cactaceae into account. Aiming to confirm these hypotheses in an evolutionary context, we obtained chromosome numbers for 20 species of Cactoideae, performed an extensive review of chromosome number for the family, and analyzed these data using a phylogenetic approach. The karyotypes presented here were characterized by CMA/DAPI banding, and for six species 5S and 45S rDNA sites were located. Our data, along with a survey of the literature, reinforce the long-standing hypothesis of a x = 11 as the base chromosome number... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Base chromosome number; CMA/DAPI; Dysploidy; Heterochromatin; Polyploidy; RDNA. |
Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062020000100135 |
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