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Production of xylanase and CMCase on solid state fermentation in different residues by Thermoascus aurantiacus miehe BJM
Silva,Roberto da; Lago,Ellen S.; Merheb,Carolina W.; Macchione,Mariana M.; Park,Yong Kun; Gomes,Eleni.
The use of waste as raw material is important for government economy and natural balance. The purpose of this work was to study the production of CMCase and xylanase by a Brazilian strain of Thermoascus aurantiacus in solid state fermentation (SSF) using different agricultural residues (wheat bran, sugarcane bagasse, orange bagasse, corncob, green grass, dried grass, sawdust and corn straw) as substrates without enrichment of the medium and characterize the crude enzymes.The study of the extracellular cellulolytic and hemicellulolytic enzymes showed that T. arantiacus is more xylanolytic than cellulolytic. The highest levels of enzymes were produced in corncob, grasses and corn straw. All the enzymes were stable at room temperature by 24 h over a broad pH...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Thermoascus aurantiacus; Xylanase; CMCase; Solid state fermentation; Agricultural residues.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300006
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Production of endoglucanase by the native strains of Streptomyces isolates in submerged fermentation BJM
Chellapandi,P.; Jani,Himanshu M..
Cellulase is a complex enzyme system, commercially produced by filamentous fungi under solid-state and submerged cultivation. It has wide applicability in textile, food and beverage industry for effective saccharification process. In this study, cellulolytic enzyme activity, particularly endoglucanase of 26 Streptomyces strains isolated from garden soil was examined, including two isolates selected on the basis of potential cellulolytic activity on Bennett's agar medium. To enhance the endoglucanase formation in broth culture, different conditions including carbon and nitrogen sources, and growth conditions were tested. The maximum endoglucanase activity (11.25-11.90 U/mL) was achieved within 72-88 h in fermentation medium containing Tween-80, followed by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endoglucanase; Streptomyces isolates; Submerged cultivation; Media optimization; Cellulase; CMCase.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000100026
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