Home Itens selecionados Provedores de dados OAI Créditos Sobre Ajuda

			Busca avançada
	Provedor de dados Anais da ABC (AABC) (1) BABT (1) BJM (1) Autor Berbara,Ricardo L.L. (1) Carvalho,Yanê (1) Covacevich,Fernanda (1) Cunha,Eunice Atsuko (1) Higuti,llma Hiroko (1) Leite,Clarice Queico Fujimura (1) Melo,Fernando Augusto Fiúza de (1) Mendes,Natália Helena (1) Miyata,Marcelo (1) Mondino,Eduardo A. (1) Moreira,Mônica (1) Neiva,Ivana Froede (1) Noschang,Juliana (1) Pimentel,João P. (1) Santos,Adolfo Carlos Barreto (1) Studdert,Guillermo A. (1) Vicente,Vânia Aparecida (1) Mais... Palavra-chave CTAB (3) Agroecosystems (1) DNA extraction (1) DNA isolation (1) Mycobacterium tuberculosis (1) Nematodes (1) Nucleic acid extraction (1) Streptococcus (1) Thermolysis (1) Ultrasound (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2015 (1) 2011 (1) 2010 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Extracting DNA of nematodes communities from Argentine Pampas agricultural soils Anais da ABC (AABC) Mondino,Eduardo A.; Covacevich,Fernanda; Studdert,Guillermo A.; Pimentel,João P.; Berbara,Ricardo L.L.. We examined four strategies (Tris/EDTA, sodium dodecyl sulfate, Chelex 100 resin and cetyltrimethylammonium bromide -CTAB-) for extracting nucleic acid (DNA) from communities of nematodes. Nematodes were isolated from an agricultural area under different management of long-term crop rotation experiment from Argentina during three seasons. After DNA extraction, Polymerase Chain Reaction-amplifications were performed and considered as indicators of successful DNA extraction. The CTAB combined with proteinase K and phenol-chloroform-isoamyl alcohol was the unique successful method because positive amplifications were obtained by using both eukaryotic and nematode specific primers. This work could contribute to biodiversity studies of nematodes on... Tipo: Info:eu-repo/semantics/article Palavras-chave: Agroecosystems; Nematodes; Nucleic acid extraction; CTAB. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200691 Methodological variations in the isolation of genomic DNA from Streptococcus bacteria BABT Moreira,Mônica; Noschang,Juliana; Neiva,Ivana Froede; Carvalho,Yanê; Higuti,llma Hiroko; Vicente,Vânia Aparecida. In this work, genomic DNA of Streptococcus pyogenes, S. mutans and S. sobrinus was isolated using two methods: either using the detergent cetyltrimethylammonium bromide (CTAB) at 65ºC; or by applying ultrasound to a mixture of silica and celite in CTAB. The composite method that used ultrasound was the more efficient, allowing the straightforward extraction of genomic DNA from Gram-positive bacteria with good quality and reproducibility. Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptococcus; DNA isolation; CTAB; Ultrasound. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400013 Assessment of the quality of dna extracted by two techniques from Mycobacterium tuberculosis for fast molecular identification and genotyping BJM Miyata,Marcelo; Santos,Adolfo Carlos Barreto; Mendes,Natália Helena; Cunha,Eunice Atsuko; Melo,Fernando Augusto Fiúza de; Leite,Clarice Queico Fujimura. We report a comparative study of two DNA extraction techniques, thermolysis and chemical lysis (CTAB), for molecular identification and genotyping of M. tuberculosis. Forty DNA samples were subjected to PCR and the results demonstrated that with thermolysis it is possible to obtain useful data that enables fast identification and genotyping. Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA extraction; Mycobacterium tuberculosis; Thermolysis; CTAB. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200045 Registros recuperados: 3 Primeira ... 1 ... Última

Empresa Brasileira de Pesquisa Agropecuária - Embrapa Todos os direitos reservados, conforme Lei n° 9.610 Política de Privacidade Área restrita		Embrapa Parque Estação Biológica - PqEB s/n° Brasília, DF - Brasil - CEP 70770-901 Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco