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Detection of respiratory viruses in shelter dogs maintained under varying environmental conditions BJM
Monteiro,Francielle Liz; Cargnelutti,Juliana Felipetto; Martins,Mathias; Anziliero,Deniz; Erhardt,Magnólia Martins; Weiblen,Rudi; Flores,Eduardo Furtado.
Abstract Three dog shelters in Rio Grande do Sul were investigated for associations between the occurrence of respiratory viruses and shelter environmental conditions. Nasal secretions randomly collected during the cold season were tested via PCR, and this data collection was followed by nucleotide sequencing of the amplicons. In shelter #1 (poor sanitary and nutritional conditions, high animal density and constant contact between dogs), 78% (58/74) of the nasal samples were positive, 35% (26/74) of which were in single infections and 44% (32/74) of which were in coinfections. Shelters #2 and #3 had satisfactory sanitary and nutritional conditions, outdoors exercise areas (#2) and animal clustering by groups (#3). In shelter #2, 9% (3/35) of the samples...
Tipo: Info:eu-repo/semantics/article Palavras-chave: CDV; CPIV; CAdV-2; CaHV-1.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400876
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Transcriptomic analysis of differential gene expression reveals an increase in COX2 levels during in vitro canine herpesvirus infection Ciência Rural
Kurissio,Jacqueline Kazue; Araújo Júnior,João Pessoa.
ABSTRACT: Canine herpesvirus (CaHV-1) affects canids worldwide, causing death in neonates and immunosuppressed hosts. Acute infection by CaHV-1 can cause reproductive, respiratory, and neurological problems in adult animals. Viral pathogenesis and host genes expressions during of CaHV-1infection are not clearly understood. In the present study, the transcriptome of canine kidney cell Mardin-Darby (MDCK) infected in vitro with canine herpesvirus was explored. For this, RNA sequencing (RNA-seq) of the samples in different moments during infection was carried out. Subsequently, the transcriptomic analysis genes related to cell activities and process involved to viral cycle infection were evaluated until 32h post-inoculation (pi). Among evaluated genes, was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Transcriptome; Herpesvirus; CaHV-1; PTGS2; COX2; Canine pathogen.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001000451
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