| The influence of the parasitism by the digenetic trematode Eurytrema coelomaticum (Giard & Billet, 1892) over the calcium mobilization and the reproductive function of its first intermediate host, the snail Bradybaena similaris (Férussac, 1821) was experimentally studied in infected and uninfected snails maintained in laboratory under controlled conditions of nutrition, humidity and temperature. The calcium of the shells of infected and uninfected snails was quantified by using a technique of volumetric complexation. The amount of calcium present in the tissue was evaluated by using the histochemistry techniques of Dahl and von Kossa. The alteration in the reproductive function was evaluated through the egg production, and by histological follow up... |
