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Influences of medium and seed age on callus formation from mature embryos of wild rice (Zizania palustris L.) OAK
村山, 廉生; 三浦, 秀穂; MURAYAMA, Yasuo; MIURA, Hideho.
Palavras-chave: 遺伝子源; カルス誘導; 胚培養; ワイルドライス; Gene resource; Callus formation; Embryo culture; Wild rice.
Ano: 1996 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1338
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Evaluation of gelling agents on anther culture: response of two soybean cultivars BABT
Cardoso,Milena Barcelos; Bodanese-Zanettini,Maria Helena; Mundstock,Elsa Cristina de; Kaltchuk-Santos,Eliane.
Anthers of two soybean cultivars were cultured in B5 long basal culture media gelled with agarose or PhytagelTM. Cytological examinations of the anthers were carried out during the first 45 days of culture to assay the viability and developmental stage of microspores. Frequency of callus formation was recorded at 45 days of culture. The analysis of variance of the microspore viability assay showed significant Cultivar X Gelling Agent X Day of Culture interactions. The frequencies of viable microspores decreased significantly with time of culture, within each cultivar and gelling agent tested. The interaction Day X Cultivar was significant for the frequencies of binucleate symmetrical grains and multinucleate/multicellular structures. The effect of gelling...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anther culture; Glycine max; Culture media; Binucleate pollen grains; Multinucleate/multicellular structures; Callus formation.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000700004
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