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Characterization and properties of the biosurfactant produced by Candida lipolytica UCP 0988 Electron. J. Biotechnol.
Diniz Rufino,Raquel; Moura de Luna,Juliana; de Campos Takaki,Galba Maria; Asfora Sarubbo,Leonie.
Background: Biotechnological processes are costly, especially for the production of biosurfactants. The successful production of a biosurfactant is dependent on the development of processes using low cost raw materials. Considering the importance of the characteristics of a biosurfactant to facilitate its industrial application, the properties of the biosurfactant produced by Candida lipolytica through previously optimized medium have been established. Results: The yeast was grown for 72 h to determine the kinetics of growth and production. The surface tension of the cell-free broth was reduced from 55 to 25 mN/m. The yield of biosurfactant was 8.0 g/l with a CMC of 0.03%. The biosurfactant was characterized as an anionic lipopeptide composed of 50%...
Tipo: Journal article Palavras-chave: Biosurfactant; Candida lipolytica; Lipopeptide; Surface tension; Toxicity.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000100006
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New bioemulsifiers produced by Candida lipolytica using D-glucose and babassu oil as carbon sources BJM
Vance-Harrop,Mabel H.; Gusmão,Norma B. de; Campos-Takaki,Galba Maria de.
Candida lipolytica IA 1055 produced extracellular biosurfactants with emulsification activity by fermentation using babassu oil and D-glucose as carbon sources. Natural seawater diluted at 50% supplemented with urea, ammonium sulfate, and phosphate was used as economic basal medium. The best results were achieved with the YSW-B2 medium, which contained urea, ammonium sulfate, and babassu oil and with YSW-B3 medium, which contained urea, ammonium sulfate, phosphate, and babassu oil, kept under fed batch fermentation for 60 hours with 5% of babassu oil. For the two media, the maximum specific growth rates were 0.02 h-1 and 0.04 h-1; the generation times were 34.6 h-1 and 17.3 h-1, and the emulsification activities were 0.666 and 0.158 units, respectively....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioemulsifier; Candida lipolytica; Seawater; Babassu oil.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200006
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A cytochemical study of acid carbohydrates on the surface of Candida lipolytica grown in tween 80-containing medium BJM
Nascimento,Aline E.; Shariá,Allana E. do Nascimento; Lima,Marco Antonio Barbosa de; Campos-Takaki,Galba Maria.
Carbohydrate-containing molecules were located on the surface of Candida lipolytica by using ruthenium red in a cytochemical study. The yeast was grown in media containing Tween 80. The surfactant, at 1.0% and 0.5%, was added to the culture medium in different intervals of time, correspondent to the beginning of exponential growth phase, mid of logarithimic phase and beginning of stationary growth phase. Control cultures were grown in a medium containing glucose. The growth of the yeast in media containing glucose and Tween 80 induced changes in the pattern of distribution and location of acid polyssacharides in the cell wall of the microorganism. In adition, the pattern also changed according to Tween 80 concentration. The influence of Tween 80 on...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida lipolytica; Carbohydrate cytochemistry; Fungi ultrastructure.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000100008
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