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| Campana,Eloiza Helena; Xavier,Danilo Elias; Petrolini,Fernanda Villas-Boas; Cordeiro-Moura,Jhonatha Rodrigo; Araujo,Maria Rita Elmor de; Gales,Ana Cristina. |
| Abstract The mechanisms involved in the uncommon resistance phenotype, carbapenem resistance and broad-spectrum cephalosporin susceptibility, were investigated in 25 Pseudomonas aeruginosa clinical isolates that exhibited this phenotype, which were recovered from three different hospitals located in São Paulo, Brazil. The antimicrobial susceptibility profile was determined by CLSI broth microdilution. β-lactamase-encoding genes were investigated by PCR followed by DNA sequencing. Carbapenem hydrolysis activity was investigated by spectrophotometer and MALDI-TOF assays. The mRNA transcription level of oprD was assessed by qRT-PCR and the outer membrane proteins profile was evaluated by SDS-PAGE. Genetic relationship among P. aeruginosa isolates was assessed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gram-negative bacilli; Carbapenem resistance; OprD porin. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000100057 |
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| Scheffer,Mara Cristina; Gales,Ana Cristina; Barth,Afonso Luis; Carmo Filho,José Rodrigues do; Dalla-Costa,Libera Maria. |
| This study evaluated the clonal spread of carbapenem-resistant P. aeruginosa producing SPM-1 type metallo-β-lactamase (MBL), at the university hospital of Florianópolis, Santa Catarina, Brazil, compared to an epidemic clone previously reported, as well as strains collected in other three Brazilian states. Among the isolates, 17 (62%) were clonal and highly related to strains from other regions of Brazil. Six clonal strains harbored the blaSPM-1 gene. The finding of a unique SPM-1 producer clone suggests that its dissemination has contributed to the high resistance to carbapenems in Brazilian hospitals |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: P. aeruginosa; Carbapenem resistance; Metallo- Β-lactamase. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000500014 |
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| Corrêa,Laís Lisboa; Botelho,Larissa Alvarenga Batista; Barbosa,Lívia Carvalho; Mattos,Claudio Simões; Carballido,Jupira Miron; Castro,Carmem Lúcia Teixeira de; Mondino,Pedro Juan Jose; Paula,Geraldo Renato de; Mondino,Silvia Susana Bona de; Mendonça-Souza,Claudia Rezende Vieira de. |
| INTRODUCTION: Acinetobacter spp. have emerged as notorious pathogens involved in healthcareassociated infections. Carbapenems are important antimicrobial agents for treating infections due to multidrug resistant Acinetobacter spp. Different mechanisms may confer resistance to these drugs in the genus, particularly production of class D carbapenemases. OXA-23-like family has been pointed out as one of the predominant carbapenamases among Acinetobacter. The present work aimed to investigate the occurrence of OXA-23-like carbapenemases among Acinetobacter isolates recovered from patients of a university hospital in Niterói, RJ, Brazil. METHODS: Antimicrobial susceptibility profiles were determined by disk-diffusion. Imipenem resistant isolates were submitted... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acinetobacter spp; OXA-23; Carbapenem resistance. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000600004 |
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| Neves,Francelli Cordeiro; Clemente,Wanessa T.; Lincopan,Nilton; Paião,Isabela D.; Neves,Patrícia R.; Romanelli,Roberta M.; Lima,Stella S.S.; Paiva,Luciene F.; Mourão,Paulo Henrique O.; Nobre-Junior,Vandack A.. |
| ABSTRACT Background: Carbapenem-resistant Acinetobacter baumannii (CRAb) is an important cause of nosocomial infections especially in intensive care units. This study aimed to assess clinical aspects and the genetic background of CRAb among ICU patients at a Brazilian teaching hospital. Methods: 56 critically ill patients colonized or infected by CRAb, during ICU stay, were prospectively assessed. Based on imipenem MIC ≥ 4 µg/mL, 28 CRAB strains were screened for the presence of genes encoding metallo-β-lactamases and OXA-type β-lactamases. The blaOXA-type genes were characterized by PCR using primers targeting ISAba-1 or -3. Genetic diversity of blaOXA-positive strains was determined by ERIC-PCR analysis. Results: Patient's mean age (±SD) was 61... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acinetobacter baumannii; Carbapenem resistance; BlaOXA-23; BlaOXA-143. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000600556 |
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| Fehlberg,Lorena C.C.; Carvalho,Albalucia M.C.; Campana,Eloiza H.; Gontijo-Filho,Paulo P.; Gales,Ana C.. |
| The emergence of KPC-2 producing K. pneumoniae in hospitalized patients at the intensive care unit (ICU) of a teaching hospital located in the city of João Pessoa, Paraíba, Brazil, is reported. Seven carbapenem-resistant K. pneumoniae recovered from different body sites of infection were analyzed. Most isolates showed a multidrug-resistance phenotype. Genotypic analysis demonstrated the presence of two genotypes, with the predominance of genotype A, which belongs to ST 437. These isolates also carry the encoding genes of five other betalactamases. |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Beta-lactam resistance; Modified Hodge test; Carbapenem resistance; Enterobacteriaceae. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000600013 |
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