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	Provedor de dados Ciênc. Tecnol. Aliment. (2) Autor BISPO,Eliete da Silva (2) SILVEIRA,Paulo Túlio de Souza (2) SOARES,Sérgio Eduardo (2) MELO,Lorena de Jesus Próculo (1) ROCHA,Fátima de Souza (1) SOUSA,Luciane Santos (1) SOUZA,Luciane Santos (1) Mais... Palavra-chave Aminopeptidase (2) Carboxypeptidase (2) Endoprotease (2) Amino acids (1) Cocoa (1) Peptide bonds (1) Proteolysis (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2017 (1) 2016 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Protease prospecion and determination of its isoenzymes activity in cocoa cultivars (Theobroma cacao L.) Ciênc. Tecnol. Aliment. SILVEIRA,Paulo Túlio de Souza; MELO,Lorena de Jesus Próculo; SOUZA,Luciane Santos; BISPO,Eliete da Silva; SOARES,Sérgio Eduardo. Abstract Our objective was to characterize the protease enzymatic activity and its isoenzymes on cacao cultivars PH 16 and TSH 1188, produced in southern Bahia, linking it to the conditions of the fermentation process. Proteases were extracted and semi-purified, their activities determined changing substrate, pH and temperature, and the values ​​compared with the parameters of fermentation (pH and temperature), and yet determined the kinetic parameters and the activity of its isoenzymes: aminopeptidase, carboxypeptidase and endoprotease. In the experimental conditions, differences in protease activities were shown as to different cocoa cultivars on various conditions. The albumin stands out as the preferred enzyme substrate, in a pH range between 3 and 6... Tipo: Info:eu-repo/semantics/article Palavras-chave: Aminopeptidase; Carboxypeptidase; Endoprotease; Peptide bonds; Amino acids. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612017000300369 Enzymatic activity of proteases and its isoenzymes in fermentation process in cultivars of cocoa (Theobroma cacao L.) produced in southern Bahia, Brazil Ciênc. Tecnol. Aliment. SOUSA,Luciane Santos; ROCHA,Fátima de Souza; SILVEIRA,Paulo Túlio de Souza; BISPO,Eliete da Silva; SOARES,Sérgio Eduardo. Abstract The fermentation of cocoa seeds envolves microbial processes and the action of enzymes. To identify the possible differences in the cocoa fermentation process, with regards to proteolysis, this study has the objective of determining protease activity (under predetermined conditions) and its isoenzymes in two cocoa cultivars (PH-16 and HRT-1188) in different cocoa fermentation times, in addition to establishing the microbial load (molds and yeasts and aerobic mesophilic). Protease and its isoenzymes were extracted and partially purified and the enzymatic activities determined by spectrophotometry. The results showed that the proteases activity was higher at 66h of fermentation for both cultivars. When the isoenzymes activity was evaluated, the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cocoa; Proteolysis; Aminopeptidase; Carboxypeptidase; Endoprotease. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612016000400656 Registros recuperados: 2 Primeira ... 1 ... Última

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