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| Lerin,Lindomar; Toniazzo,Geciane; Oliveira,Débora de; Rottava,Leda; Dariva,Cláudio; Cansian,Rogério Luis; Treichel,Helen; Padilha,Francine; Antunes,Octávio Augusto Ceva. |
| Limonene is a monoterpene obtained in large amounts from essential oils and is used as a raw material for the synthesis of flavors and fine chemicals. Several pathways or routes for the microbial degradation of limonene making use of the cytochrome P450-dependent monooxygenases have been described. In this study, we present a fermentative screening of microorganisms in order to verify their ability to perform the desirable conversion. In parallel, the PCR technique was used to select the microorganisms that contain the limC gene, which is responsible for the conversion of carveol to carvone. The microorganisms selected by PCR were not able to bioconvert limonene. From this result, we can suppose that these strains do not have the gene that codifies the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: CDH (carveol dependent dehydrogenase); Carveol; Carvone; PCR; Fermentative screening. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612010000200017 |
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| La Rotta Hernández,Camilo Enrique; Werberich,Diogo Simon; Mattos,Marcio Contrucci Saraiva de; D'Elia,Eliane. |
| Horse radish peroxidase (HRP) from Armoracia rusticana catalyses the oxidation of (R)-limonene into the oxidized derivatives carveol and carvone. This study compares the direct addition (DA) of hydrogen peroxide with its continuous electrogeneration (EG) during the enzymatic oxidation of (R)-limonene. Reaction mixtures containing HRP, (R)-limonene as substrate, and hydrogen peroxide, added directly or electrogenerated, in 100 mM sodium-potassium phosphate buffer pH 7.0, at 25ºC were studied. Two electrochemical systems for the hydrogen peroxide electrogeneration were evaluated, both containing as auxiliary electrode (AE) a platinum wire and saturated calomel electrode (SCE) as reference. Reticulated vitreous carbon foam (RVCF) and an electrolytic copper... |
| Tipo: Journal article |
Palavras-chave: Armoracia rusticana; Bioelectrochemistry; Carveol; Carvone; Horse-radish peroxidase; Hydrogen peroxide electrogeneration; (R)-limonene. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000400006 |
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