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	Provedor de dados BJMBR (1) Autor Anziliero,D. (1) Brum,M.C.S. (1) Flores,E.F. (1) Weiblen,R. (1) Weiss,M. (1) Palavra-chave Cattle pathogen (1) Control (1) Differential vaccine (1) Immunization (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2015 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última A glycoprotein E gene-deleted bovine herpesvirus 1 as a candidate vaccine strain BJMBR Weiss,M.; Brum,M.C.S.; Anziliero,D.; Weiblen,R.; Flores,E.F.. A bovine herpesvirus 1 (BoHV-1) defective in glycoprotein E (gE) was constructed from a Brazilian genital BoHV-1 isolate, by replacing the full gE coding region with the green fluorescent protein (GFP) gene for selection. Upon co-transfection of MDBK cells with genomic viral DNA plus the GFP-bearing gE-deletion plasmid, three fluorescent recombinant clones were obtained out of approximately 5000 viral plaques. Deletion of the gE gene and the presence of the GFP marker in the genome of recombinant viruses were confirmed by PCR. Despite forming smaller plaques, the BoHV-1△gE recombinants replicated in MDBK cells with similar kinetics and to similar titers to that of the parental virus (SV56/90), demonstrating that the gE deletion had no deleterious effects... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cattle pathogen; Immunization; Control; Differential vaccine. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000900843 Registros recuperados: 1 Primeira ... 1 ... Última

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