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	Provedor de dados Electron. J. Biotechnol. (1) Rev. Bras. Ciênc. Avic. (1) Autor Arenas,Gloria (1) Bai,J (1) Díaz,Mauricio (1) Feng,J (1) Marchetti,Stefano (1) Marshall,Sergio H (1) Venturini,Elena (1) Wang,R (1) Yan,L (1) Mais... Palavra-chave Cecropin (2) Antimicrobial (1) Broiler (1) Fusion (1) Intein (1) Laminaria japonica powder (1) Live Performance (1) Peptide (1) Soluble (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Journal article (1) Ano 2019 (1) 2012 (1) País Brazil (1) Chile (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Intein-mediated expression of cecropin in Escherichia coli Electron. J. Biotechnol. Díaz,Mauricio; Venturini,Elena; Marchetti,Stefano; Arenas,Gloria; Marshall,Sergio H. Different strategies have been used to overcome the difficulties to produce antimicrobial peptides. Here we used Intein Mediated Purification with an Affinity Chitin-binding Tag (IMPACT-System, New England Biolabs) for the expression of the antimicrobial peptide cecropin to reduce its sensitivity to intracellular proteases and use its inducible self-cleaving capability to remove the carrier. Cecropin was cloned into suitable expression vector pTYB11, and expression induced by IPTG in Escherichia coli ER2566. The use of 22ºC induction allowed the expression of cecropin with its intein carrier in soluble form. Cell extracts were purified by chitin affinity chromatography and intein-mediated splicing of the target protein was achieved by thiol addition,... Tipo: Journal article Palavras-chave: Antimicrobial; Cecropin; Fusion; Intein; Peptide; Soluble. Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200003 Co-Supplementation of Dietary Seaweed Powder and Antibacterial Peptides Improves Broiler Growth Performance and Immune Function Rev. Bras. Ciênc. Avic. Bai,J; Wang,R; Yan,L; Feng,J. ABSTRACT The feasibility of Laminaria japonica powder (LJP) combined with cecropin as a dietary supplement to enhance broiler growth performance and immune function was evaluated in this study. In total, 648 one-day-old Arbor Acres broiler chicks were randomly distributed into nine numerically-equal treatment groups: T1 (control group; fed a basal diet); T2 (fed the basal diet supplemented with 1% LJP);T3 (fed the basal diet supplemented with 300mg cecropin/kg); and T4,T5,T6,T7,T8 and T9, individually fed with the dietary supplemented with varying levels of LJP and cecropin). Compared with the control, dietary of LJP or cecropin supplementation slightly improved feed conversion ratio (FCR). However, the dietary supplementation of LJP combined with cecropin... Tipo: Info:eu-repo/semantics/article Palavras-chave: Broiler; Cecropin; Laminaria japonica powder; Live Performance. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000200308 Registros recuperados: 2 Primeira ... 1 ... Última

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