SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Moreira,Maria Elisabete C.; Barcinski,Marcello A..

Cell death by apoptosis is characterized by specific biochemical changes, including the exposure of multiple ligands, expected to tag the dying cell for prompt recognition by phagocytes. In non-pathological conditions, an efficient clearance is assured by the redundant interaction between apoptotic cell ligands and multiple receptor molecules present on the engulfing cell surface. This review concentrates on the molecular interactions operating in mammalian and non-mammalian systems for apoptotic cell recognition, as well as on the consequences of their signaling. Furthermore, some cellular models where the exposure of the phosphatidylserine (PS) phospholipid, a classical hallmark of the apoptotic phenotype, is not followed by cell death will be discussed.

The Complex Link between Apoptosis and Autophagy: a Promising New Role for RB

Palavras-chave: Cell death; Apoptosis; Apoptotic cell recognition; Apoptotic mimicry; Phagocyte; Intracellular signaling.

Ano: 2004

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652004000100009

DELOU,JOÃO M.A.; BIASOLI,DEBORAH; BORGES,HELENA L..

ABSTRACT Physiological processes, as autophagy, proliferation and apoptosis are affected during carcinogenesis. Restoring cellular sensitivity to apoptotic stimuli, such as the antineoplastic cocktails, has been explored as a strategy to eliminate cancer cells. Autophagy, a physiological process of recycling organelles and macromolecules can be deviated from homeostasis to support cancer cells survival, proliferation, escape from apoptosis, and therapy resistance. The relationship between autophagy and apoptosis is complex and many stimuli can induce both processes. Most chemotherapeutic agents induce autophagy and it is not clear whether and how this chemotherapy-induced autophagy might contribute to resistance to apoptosis. Here, we review current...

"In vivo" toxicity of a truncated version of the Drosophila Rst-IrreC protein is dependent on the presence of a glutamine-rich region in its intracellular domain

Palavras-chave: Retinoblastoma; Neoplasm; Autophagy; Cell death; Antineoplastic agents.

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000602257

MACHADO,RICARDO C.; PEREIRA,RODRIGO N.R.; COSTA,MARA S.A.; RAMOS,RICARDO GUELERMAN P..

The roughest-irregular chiasm C ( rst-irreC) gene of Drosophila melanogaster encodes a transmembrane glycoprotein containing five immunoglobulin-like domains in its extracellular portion and an intracytoplasmic tail rich in serine and threonine as well some conserved motifs suggesting signal transduction activity. In the compound eye, loss-of-function rst-irreC mutants lack the characteristic wave of programmed cell death happening in early pupa and which is essential for the elimination of the surplus interommatidial cells. Here we report an investigation on the role played by the Rst-irreC molecule in triggering programmed cell death. "In vivo" transient expression assays showed that deletion of the last 80 amino acids of the carboxyl terminus produces a...

High susceptibility of activated lymphocytes to oxidative stress-induced cell death

Palavras-chave: Drosophila; Rst-irreC; Glutamine-rich repeats; Cell death.

Ano: 2002

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652002000200007

Degasperi,Giovanna R.; Castilho,Roger F.; Vercesi,Anibal E..

The present study provides evidence that activated spleen lymphocytes from Walker 256 tumor bearing rats are more susceptible than controls to tert-butyl hydroperoxide (t-BOOH)-induced necrotic cell death in vitro. The iron chelator and antioxidant deferoxamine, the intracellular Ca2+ chelator BAPTA, the L-type Ca2+ channel antagonist nifedipine or the mitochondrial permeability transition inhibitor cyclosporin A, but not the calcineurin inhibitor FK-506, render control and activated lymphocytes equally resistant to the toxic effects of t-BOOH. Incubation of activated lymphocytes in the presence of t-BOOH resulted in a cyclosporin A-sensitive decrease in mitochondrial membrane potential. These results indicate that the higher cytosolic Ca2+ level in...

Apoptosis in the transplanted canine transmissible venereal tumor during growth and regression phases

Palavras-chave: Cell death; Free radicals; Immune response; Lymphopenia; Mitochondrial permeability transition; Spleen lymphocyte.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652008000100009

Arq. Bras. Med. Vet. Zootec.

Santos,F.G.A.; Vasconcelos,A.C.; Nunes,J.E.S.; Cassali,G.D.; Paixão,T.A.; Martins,A.S.; Silva,S.S.; Martins,R.F.; Moro,L..

Twelve male, mongrel, adult dogs were subcutaneously transplanted with cells originated from two canine transmissible venereal tumors (TVT). The aim was to demonstrate and to quantify the occurrence of apoptosis in the TVT regression. After six months of transplantation, a tumor sample was obtained from each dog, being six dogs with TVT in the growing phase and six in the regression phase as verified by daily measurements. Samples were processed for histological and ultrastructural purposes as well as for DNA extraction. Sections of 4µm were stained by HE, Shorr, methyl green pyronine, Van Gieson, TUNEL reaction and immunostained for P53. The Shorr stained sections went through morphometry that demonstrated an increase of the apoptotic cells per field in...

Autophagy, apoptosis and organelle features during cell exposure to cadmiumc

Palavras-chave: Dog; Venereal tumor; Apoptosis; Cell death.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000300013

Biocell

dos Santos Vergilio,Cristiane; Tenório de Melo,Edésio José.

Cadmium (Cd) induces several effects in different tissues, but our knowledge of the toxic effects on organelles is insufficient. To observe the progression of Cd effects on organelle structure and function, HuH-7 cells (human hepatic carcinoma cell line) were exposed to CdCl2 in increasing concentrations (1 μM - 20 μM) and exposure times (2 h - 24 h). During Cd treatment, the cells exhibited a progressive decrease in viability that was both time- and dose-dependent. Cd treated cells displayed progressive morphological changes that included cytoplasm retraction and nuclear condensation preceding a total loss of cell adhesion. Treatment with 10 μM for 12 h led to irreversible damages. Before these drastic and irreparable damages,...

URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452013000200005

Palavras-chave: Cd; Cell death; Hepatocyte; HuH-7 cells; Mitochondria.

Ano: 2013

Ultrastructural effects of two phthalocyanines in CHO-K1 and HeLa cells after laser irradiation

Biocell

de CastroPazos,Marcelo; Pacheco-Soares,Cristina; Soares da Silva,Newton; DaMatta,Renato Augusto; Pacheco,Marcos Tadeu T..

The effects of Photodynamic Therapy using 2nd generation photosensitizers have been widely investigated aiming clinical application treatment of solid neoplasms. In this work, ultrastructure changes caused by the action of two 2nd generation photosensitizers and laser irradiation on CHO-K1 and HeLa (neoplastic) cells were analyzed by transmission electron microscopy. Aluminum phthalocyanine chloride, aluminum phthalocyanine tetrasulfonate chloride and radiation from a semiconductor laser at a fluency of 0.5 J/cm² (Power=26mW; l=670nm) were used. The results showed induction of apoptosis. Such alterations where observed in HeLa but not in CHO-K1 cells after Aluminum phthalocyanine tetrasulfonate chloride (AlPcS4) photodynamic treatment. The Aluminum...

URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000300001

Palavras-chave: Photodynamic therapy; Phthalocyanines; Laser; Cell death; Ultrastructure.

Ano: 2003

Suppression of miR-181 a attenuates H2O2-induced death of mesenchymal stem cells by maintaining hexokinase II expression

Antioxidant responses of cortex neurons to iron loading

Lee,Seahyoung; Yun,Ina; Ham,Onju; Lee,Se-Yeon; Lee,Chang Yeon; Park,Jun-Hee; Lee,Jiyun; Seo,Hyang-Hee; Choi,Eunhyun; Hwang,Ki-Chul.

BACKGROUND: Low survival rate of transplanted cells compromises the efficacy of cell therapy. Hexokinase II (HKII) is known to have anti-apoptotic activity through its interaction with mitochondria. The objective was to identify miRNAs targeting HKII and investigate whether miRNA-mediated modulation of HKII could improve the survival of mesenchymal stem cells (MSCs) exposed to H2O2. The expression of HKII in MSCs exposed to H2O2 was evaluated, and HKII-targeting miRNA was screened based on miRNA-target prediction databases. The effect of H2O2 on the expression of the selected HKII-targeting miRNA was examined and the effect of modulation of the selected HKII-targeting miRNA using anti-miRNA on H2O2-induced apoptosis of MSC was evaluated. RESULTS: H2O2 (600...

Tipo: Journal article

Palavras-chave: Hexokinase II; MiRNA-181a; Cell death; Mesenchymal stem cell.

Ano: 2015

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100045

Non-selective cation channels and oxidative stress- induced cell swelling

AGUIRRE,PABLA; MENA,NATALIA; TAPIA,VICTORIA; ROJAS,ALEJANDRO; ARREDONDO,MIGUEL; NÚÑEZ,MARCO TULIO.

Brain cells have a highly active oxidative metabolism, yet they contain only low to moderate superoxide dismutase and catalase activities. Thus, their antioxidant defenses rely mainly on cellular reduced glutathione levels. In this work, in cortical neurons we characterized viability and changes in reduced and oxidized glutathione levels in response to a protocol of iron accumulation. We found that massive death occurred after 2 days in culture with 10 mM Fe. Surviving cells developed an adaptative response that included increased synthesis of GSH and the maintenance of a glutathione-based reduction potential. These results highlight the fundamental role of glutathione homeostasis in the antioxidant response and provide novel insights into the adaptative...

Tipo: Journal article

Palavras-chave: Glutathione; Iron; Redox response; Cortical neurons; Cell death.

Ano: 2006

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100012

Ion movements in cell death: from protection to execution

SIMON,FELIPE; VARELA,DIEGO; RIVEROS,ANA; EGUIGUREN,ANA LUISA; STUTZIN,ANDRES.

Necrosis is considered as a non-specific form of cell death that induces tissue inflammation and is preceded by cell swelling. This increase in cell volume has been ascribed mainly to defective outward pumping of Na+ caused by metabolic depletion and/or to increased Na+ influx via membrane transporters. A specific mechanism of swelling and necrosis driven by the influx of Na+ through nonselective cation channels has been recently proposed (<A HREF="#2">Barros et al., 2001a</A>). We have characterized further the properties of the nonselective cation channel (NSCC) in HTC cells. The NSCC shows a conductance of ~18 pS, is equally permeable to Na+ and K+, impermeant to Ca2+, requires high intracellular Ca2+ as well as low intracellular ATP for...

Tipo: Journal article

Palavras-chave: Cell death; Cell Volume; Nonselective cation channels; Flufenamic acid.

Ano: 2002

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200013

FELIPE BARROS,LUIS; CASTRO,JOEL; BITTNER,CARLA X..

Cell death is preceded by severe disruption of inorganic ion homeostasis. Seconds to minutes after an injury, calcium, protons, sodium, potassium and chloride are exchanged between the cell and its environment. Simultaneously, ions are shifted between membrane compartments inside the cell, whereby mitochondria and endoplasmic reticulum play a crucial role. Depending of the type and severity of injury, two mutually exclusive metastable states can be reached, which predict the final outcome. Cells characterized by large increases in cytosolic [Ca2+], [Na+]; and [Mg2+] swell and die by necrosis; alternatively, cells characterized by high [H+]and low [K+], with normal [Na+] and normal to moderate [Ca2+] increases die by apoptosis. The levels of these ions...

Tipo: Journal article

Palavras-chave: Cell death; Membrane channels; Apoptosis; Necrosis.

Ano: 2002

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200012

Oligodendrocytes damage in Alzheimer's disease: Beta amyloid toxicity and inflammation

ROTH,ALEJANDRO D; RAMÍREZ,GIGLIOLA; ALARCÓN,RODRIGO; VON BERNHARDI,ROMMY.

Research on Alzheimer's disease (AD) focuses mainly on neuronal death and synaptic impairment induced by β-Amyloid peptide (Aβ), events at least partially mediated by astrocyte and microglia activation. However, substantial white matter damage and its consequences on brain function warrant the study of oligodendrocytes participation in the pathogenesis and progression of AD. Here, we analyze reports on oligodendrocytes' compromise in AD and discuss some experimental data indicative of Aβ toxicity in culture. We observed that 1 μM of fibrilogenic Aβ peptide damages oligodendrocytes in vitro; while pro-inflammatory molecules (1 μg/ml LPS + 1 ng/ml IFNγ) or the presence of astrocytes reduced the...

Tipo: Journal article

Palavras-chave: Cell death; Glial cells; Pro-inflammatory cytokines.

Ano: 2005

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000400011

Arctiin blocks hydrogen peroxide-induced senescence and cell death though microRNA expression changes in human dermal papilla cells

Oxidative stress action in cellular aging

Bae,Seunghee; Lim,Kyung Mi; Cha,Hwa Jun; An,In-Sook; Lee,Jeong Pyo; Lee,Kwang Sik; Lee,Ghang Tai; Lee,Kun Kook; Jung,Ho Jung; Ahn,Kyu Joong; An,Sungkwan.

BACKGROUND: Accumulating evidence indicates that reactive oxygen species (ROS) are an important etiological factor for the induction of dermal papilla cell senescence and hair loss, which is also known alopecia. Arctiin is an active lignin isolated from Arctium lappa and has anti-inflammation, anti-microbial, and anti-carcinogenic effects. In the present study, we found that arctiin exerts anti-oxidative effects on human hair dermal papilla cells (HHDPCs). RESULTS: To better understand the mechanism, we analyzed the level of hydrogen peroxide (H2O2)-induced cytotoxicity, cell death, ROS production and senescence after arctiin pretreatment of HHDPCs. The results showed that arctiin pretreatment significantly inhibited the H2O2-induced reduction in cell...

Tipo: Journal article

Palavras-chave: Dermal papilla cell; Senescence; Cell death; MicroRNA; Arctiin.

Ano: 2014

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100073

BABT

Oliveira,Monique Cristine de; Schoffen,João Paulo Ferreira.

Various theories try to explain the biological aging by changing the functions and structure of organic systems and cells. During lifetime, free radicals in the oxidative stress lead to lipid peroxidation of cellular membranes, homeostasis imbalance, chemical residues formation, gene mutations in DNA, dysfunction of certain organelles, and the arise of diseases due to cell death and/or injury. This review describes the action of oxidative stress in the cells aging process, emphasizing the factors such as cellular oxidative damage, its consequences and the main protective measures taken to prevent or delay this process. Tests with antioxidants: vitamins A, E and C, flavonoids, carotenoids and minerals, the practice of caloric restriction and physical...

Salmonella enterica serovar Typhi plasmid pR ST98 enhances intracellular bacterial growth and S. typhi-induced macrophage cell death by suppressing autophagy

Palavras-chave: Antioxidants; Cellular aging; Cell death; Oxidative stress.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600009

BJID

He,Peiyan; Wu,Shuyan; Chu,Yuanyuan; Yang,Yanru; Li,Yuanyuan; Huang,Rui.

OBJECTIVES: Plasmid pR ST98 is a hybrid resistance-virulence plasmid isolated from Salmonella enterica serovar Typhi (S. typhi). Previous studies demonstrated that pR ST98 could enhance the virulence of its host bacteria. However, the mechanism of pR ST98-increased bacterial virulence is still not fully elucidated. This study was designed to gain further insight into the roles of pR ST98 in host responses. METHODS: Human-derived macrophage-like cell line THP-1 was infected with wild-type (ST8), pR ST98-deletion (ST8-ΔpR ST98), and complemented (ST8-c-pR ST98) S. typhi strains. Macrophage autophagy was performed by extracting the membrane-unbound LC3-I protein from cells, followed by flow cytometric detection of the membrane-associated fraction of LC3-II....

Effects of gamma-radiation on cell growth, cycle arrest, death, and superoxide dismutase expression by DU 145 human prostate cancer cells

Palavras-chave: Plasmid pR ST98; Macrophage; Autophagy; Intracellular bacterial growth; Cell death.

Ano: 2012

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000300008

Vucic,V.; Isenovic,E.R.; Adzic,M.; Ruzdijic,S.; Radojcic,M.B..

Gamma-irradiation (gamma-IR) is extensively used in the treatment of hormone-resistant prostate carcinoma. The objective of the present study was to investigate the effects of 60Co gamma-IR on the growth, cell cycle arrest and cell death of the human prostate cancer cell line DU 145. The viability of DU 145 cells was measured by the Trypan blue exclusion assay and the 3(4,5-dimethylthiazol-2-yl)-2,5,diphenyltetrazolium bromide test. Bromodeoxyuridine incorporation was used for the determination of cell proliferation. Cell cycle arrest and cell death were analyzed by flow cytometry. Superoxide dismutase (SOD), specifically CuZnSOD and MnSOD protein expression, after 10 Gy gamma-IR, was determined by Western immunoblotting analysis. gamma-IR treatment had a...

Detection of early apoptosis and cell death in T CD4+ and CD8+ cells from lesions of patients with localized cutaneous leishmaniasis

Palavras-chave: Gamma-irradiation; Prostate cancer; Proliferation; Cell death; Cell cycle arrest; Superoxide dismutase.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000200009

Bertho,A.L.; Santiago,M.A.; Da-Cruz,A.M.; Coutinho,S.G..

Human localized cutaneous leishmaniasis (LCL), induced by Leishmania braziliensis, ranges from a clinically mild, self-healing disease with localized cutaneous lesions to severe forms which can present secondary metastatic lesions. The T cell-mediated immune response is extremely important to define the outcome of the disease; however, the underlying mechanisms involved are not fully understood. A flow cytometric analysis of incorporation of 7-amino actinomycin D and CD4+ or CD8+ T cell surface phenotyping was used to determine whether different frequencies of early apoptosis or accidental cell death occur at different stages of LCL lesions. When all cells obtained from a biopsy sample were analyzed, larger numbers of early apoptotic and dead cells were...

Cellular and molecular studies of the effects of a selective COX-2 inhibitor celecoxib in the cardiac cell line H9c2 and their correlation with death mechanisms

Palavras-chave: Apoptosis; Flow cytometry; Human leishmaniasis; Cell death; T cells.

Ano: 2000

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000300010

Sakane,K.K.; Monteiro,C.J.; Silva,W.; Silva,A.R.; Santos,P.M.; Lima,K.F.; Moraes,K.C.M..

Cardiovascular disease is one of the leading causes of death worldwide, and evidence indicates a correlation between the inflammatory process and cardiac dysfunction. Selective inhibitors of cyclooxygenase-2 (COX-2) enzyme are not recommended for long-term use because of potentially severe side effects to the heart. Considering this and the frequent prescribing of commercial celecoxib, the present study analyzed cellular and molecular effects of 1 and 10 µM celecoxib in a cell culture model. After a 24-h incubation, celecoxib reduced cell viability in a dose-dependent manner as also demonstrated in MTT assays. Furthermore, reverse transcription-polymerase chain reaction analysis showed that the drug modulated the expression level of genes related to death...

The regulation of apoptotic cell death

Palavras-chave: Cellular and molecular analyses; Cell death; Cyclooxygenase-2 inhibitor; H9c2 cardiac cell line.

Ano: 2014

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000100050

Amarante-Mendes,G.P.; Green,D.R..

Apoptosis is a fundamental biological phenomenon in which the death of a cell is genetically and biochemically regulated. Different molecules are involved in the regulation of the apoptotic process. Death receptors, coupled to distinct members of the caspases as well as other adapter molecules, are involved in the initiation of the stress signals (The Indictment). Members of the Bcl-2 family control at the mitochondrial level the decision between life and death (The Judgement). The effector caspases are responsible for all morphological and biochemical changes related to apoptosis including the "eat-me" signals perceived by phagocytes and neighboring cells (The Execution). Finally, apoptosis would have little biological significance without the recognition...

High expression of the circadian gene mPer2 diminishes the radiosensitivity of NIH 3T3 cells

Palavras-chave: Apoptosis; Cell death; Caspase; Mitochondria; Bcl-2; Oncogene.

Ano: 1999

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900001

Chang,L.; Liu,Y.Y.; Zhu,B.; Li,Y.; Hua,H.; Wang,Y.H.; Zhang,J.; Jiang,Z.; Wang,Z.R..

Period2 is a core circadian gene, which not only maintains the circadian rhythm of cells but also regulates some organic functions. We investigated the effects of mPeriod2 (mPer2) expression on radiosensitivity in normal mouse cells exposed to 60Co-γ-rays. NIH 3T3 cells were treated with 12-O-tetradecanoylphorbol-13-acetate (TPA) to induce endogenous mPer2 expression or transfected with pcDNA3.1(+)-mPer2 and irradiated with 60Co-γ-rays, and then analyzed by several methods such as flow cytometry, colony formation assay, RT-PCR, and immunohistochemistry. Flow cytometry and colony formation assay revealed that irradiated NIH 3T3 cells expressing high levels of mPer2 showed a lower death rate (TPA: 24 h 4.3% vs 12 h 6.8% and control 9.4%; transfection:...