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Registros recuperados: 6
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Enhancement of chitosanase production by cell immobilization of Gongronella sp. JG BJM
Zhang,Pingping; Zhou,Wei; Wang,Peng; Wang,Li; Tang,Mingli.
Chitosanase production of Gongronella sp. JG cells immobilized in calcium alginate gel and polyurethane foam was compared with that of the free cells, there was a 60% increase in the enzyme yield (2429 U/L) compared to the highest yield obtained from free cells (1513 U/L). The optimal immobilization parameters (concentrations of sodium alginate, calcium chloride, bead inoculums, bead diameter, etc) for the enhanced production of chitosanase were determined as: sodium alginate 2% (w/v), 0.1 M calcium chloride, inoculum 10 mL beads to 100 mL production media and 2.7 mm bead diameter. Maximum chitosanase production was achieved with initial pH of 5.5 and temperature of 30 ºC. The alginate beads had well stability, retained 85% ability of enzyme production...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitosanase production; Gongronella sp. JG; Cell immobilization; Sodium alginate.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100029
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Glucosyltransferase production by Klebsiella sp. K18 and conversion of sucrose to palatinose using immobilized cells BJM
Orsi,Daniela C.; Kawaguti,Haroldo Y.; Sato,Hélia H..
The strain Klebsiella sp. K18 produces the enzyme glucosyltransferase and catalyses the conversion of sucrose to palatinose, an alternative sugar that presents low cariogenicity. Response Surface Methodology was successfully employed to determine the optimal concentration of culture medium components. Maximum glucosyltransferase production (21.78 U mL-1) was achieved using the optimized medium composed by sugar cane molasses (80 g L-1), bacteriological peptone (7 g L-1) and yeast extract (20 g L-1), after 8 hours of fermentation at 28°C. The conversion of sucrose to palatinose was studied utilizing immobilized cells in calcium alginate. The effects of the alginate concentration (2-4%), cell mass concentration (20-40%) and substrate concentration (25-45%)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cell immobilization; Glucosyltransferase; Klebsiella sp; Palatinose; Response surface methodology.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000100010
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Ethanol fermentation of sugarcane molasses by Zymomonas mobilis MTCC 92 immobilized in Luffa cylindrica L. sponge discs and Ca-alginate matrices BJM
Behera,Shuvashish; Mohanty,Rama C.; Ray,Ramesh C..
Bio-ethanol production from cane molasses (diluted to 15 % sugar w/v) was studied using the bacterium, Zymomonas mobilis MTCC 92 entrapped in luffa (Luffa cylindrica L.) sponge discs and Ca-alginate gel beads as the immobilizing matrices. At the end of 96 h fermentation, the final ethanol concentrations were 58.7 ± 0.09 and 59.1 ± 0.08 g/l molasses with luffa and Ca-alginate entrapped Z. mobilis cells, respectively exhibiting 83.25 ± 0.03 and 84.6 ± 0.02 % sugar conversion. There was no statistical significant difference (Fischer's LSD) in sugar utilization (t = 0.254, p <0.801) and ethanol production (t =-0.663, p <0.513) between the two immobilization matrices used. Further, the immobilized cells in both the matrices were physiologically active for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bio-ethanol; Cell immobilization; Fermentation; Molasses; Zymomonas mobilis MTCC 92.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400034
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Biodegradation kinetics of o-cresol by Pseudomonas putida DSM 548 (pJP4) and o-cresol removal in a batch-recirculation bioreactor system Electron. J. Biotechnol.
Kaymaz,Yasin; Babaoglu,Anil; Pazarlioglu,Nurdan Kasikara.
The biodegradation kinetics of o-cresol was examined by acclimatized P. putida DSM 548 (pJP4) in batch experiments at varying initial o-cresol concentrations (from 50 to 500 mg/L). The kinetic parameters of o-cresol aerobic biodegradation were estimated by using the Haldane substrate inhibition equation. The biodegradation kinetics of o-cresol was investigated. In batch culture reactors, the Maximum specific growth rate (&#956;max), Monod constant (Ks) and the inhibition constant (Ki) were established as 0.519 h-1, 223.84 mg/L and 130.883 mg/L, respectively. o-cresol biodegradation in a batch-recirculation bioreactor system by immobilized P. putida was also studied. The recycled packed bed reactor system, which was composed of Ca-alginate beads and...
Tipo: Journal article Palavras-chave: Biodegradation; Cell immobilization; O-cresol; Pseudomonas putida; Substrate inhibition kinetics.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000100003
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Relationship between cultivation mode of white rot fungi and their efficiency for olive oil mill wastewaters treatment Electron. J. Biotechnol.
Alaoui,Sanae Mdaghri; Merzouki,Mohamed; Penninckx,Michel J; Benlemlih,Mohamed.
Four white rot fungi (WRF) strains, Phanerochaete chrysosporium, Trametes versicolor, Coriolopsis polyzona and Pycnoporus coccineus, were tested for efficiency of treatment of Olive Oil mill wastewaters (OOMW) in relation with their cultivation mode, i.e. under the form of free mycelium, mycelium immobilized in alginate beads and solid state cultivation on Petri dishes. Study of biodegradation of phenolic compounds, chemical oxygen demand (COD) decrease and decolourisation of OOMW have shown that Coriolopsis polyzona and Pycnoporus coccineus degradation performances were apparently only slightly affected by the cell cultivation procedures experienced here. In contrast, Phanerochaete chrysosporium and Trametes versicolor showed respectively marked...
Tipo: Journal article Palavras-chave: Biotreatment; Cell immobilization; Olive oil mill wastewaters; White rot fungi.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000400013
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Immobilisation et culture continue en bioréacteur gas-lift de microorganismes marins thermophiles et hyperthermophiles anaérobies ArchiMer
Landreau, Matthieu.
Since the discovery of hydrothermal vents, multiple studies have been conducted in order to study microbial diversity. Molecular inventories realized have thus demonstrated a great diversity of species that contrasts with the low proportion (1%) of species isolated by culture approach. A new cell immobilization approach by inclusion in a polymer matrix (gellan and xanthan) has been developed for the study of these thermophilic anaerobic marine communities. The system, based on the formation of an emulsion between an inoculated polymer solution and oil, allows the entrapment of cells in gel beads with a diameter between 1 and 2 mm. The optimal immobilization conditions were obtained for emulsion performed at 80 °C with stirring (150 rpm) with a polymer...
Tipo: Text Palavras-chave: Immobilisation cellulaire; Gellane; Xanthane; Site Rainbow; Culture continue; Cell immobilization; Gellan; Xanthan; Rainbow site; (hyper)thermophile; Continuous culture.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00498/60933/64327.pdf
Registros recuperados: 6
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