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Donnees cytologiques, electrophysiologiques et biochimiques relatives au phenomene de fecondation chez le homard (Homarus gammarus ). Implication du processus d'activation sur le mecanisme d'attache des oeufs pondus ArchiMer
Goudeau, M; Lachaise, F; Kubisz, P; Goudeau, H.
Electrophysiological measurements and related cytological controls were done on immature and submature oocytes of Homarus gammarus . By chromatography (HPLC) and radio-immunoassays (RIA) it was established that submature oocytes displayed different ecdysteroids. Furthermore, the structure of the stalk (funiculus) for the laid eggs attachment was analyzed. it consisted of the two superimposed vitelline envelopes which were likely modified by the cortical reaction exudate during the fertilization process. In addition, the successive secretory phases, regarding to the embryonic envelopes were evidenced. Finally, the ecdysteroid titers of embryonic stages were measured.
Tipo: Text Palavras-chave: Homarus gammarus; Malacostraca; Biological attachment; Hormones; Electrophysiology; Biochemistry; Cytology; Eggs; Embryonic development; Cell membranes; Oocytes; Fecundity; Homard; Decapode; Crustacé; Funicule; Mues embryonnaires; Enveloppe de fécondation; Ecdystéroîdes; Potentiel membranaire; Ovocyte.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1190.pdf
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Isolement et caracterisation d'une proteine membranaire ovocytaire capable de fixer specifiquement la vitellogenine ArchiMer
Jugan, P; Vanherp, F.
After solubilization of ovocyte-membranes from the crayfish Orconectes limosus , a component with a molecular weight of 30 kDa and capable to fix vitellogenin specifically, could be characterized by SDS-PAGE and by electroblotting. Because this isolated component is sensible to the digestion of proteolytic enzymes such as pronase, it is assumed that it is a protein. A polyclonal antiserum is produced against this component and its tissue-specificity is verified by ELISA.
Tipo: Text Palavras-chave: Orconectes limosus; Malacostraca; Oogenesis; Biochemical analysis; Cell membranes.
Ano: 1987 URL: http://archimer.ifremer.fr/doc/1987/acte-1382.pdf
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