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	Provedor de dados Biol. Res. (1) BJMBR (1) Autor Bustamante,J.O. (1) CÁRDENAS,CÉSAR (1) HIDALGO,JORGE (1) JAIMOVICH,ENRIQUE (1) KUSNIER,CARLOS (1) Varanda,W.A. (1) Mais... Palavra-chave Cell nucleus (2) Gene activity (1) Gene expression (1) IP3 receptor (1) Ion channels (1) Macromolecular transport (1) Measurement (1) Muscle (1) Nuclear envelope (1) Nuclear pores (1) Nucleocytoplasmic transport (1) Patch-clamp (1) Single channel (1) Transcriptional control (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Journal article (1) Ano 2006 (1) 1998 (1) País Brazil (1) Chile (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Single-channel recording of inositol trisphosphate receptor in the isolated nucleus of a muscle cell line Biol. Res. KUSNIER,CARLOS; CÁRDENAS,CÉSAR; HIDALGO,JORGE; JAIMOVICH,ENRIQUE. Nuclear calcium appears to have an important role in the regulation of gene expression in many cells, but the mechanisms involved in controlling nuclear Ca2+ signaling are controversial and still poorly understood. We have described the presence of inositol 1,4,5 trisphosphate (IP3) receptors in the nuclei of skeletal muscle cells. Now, we have characterized the properties of the IP3 receptors channels present in the nuclei of the 1B5 cell line, which do not express any isoforms of the ryanodine receptor. Immunocytochemistry of isolated nuclei confirmed the presence of IP3R in the nuclear envelope and fluorescence measurements in nuclei suspensions allowed us to document ATP-dependent calcium loading by the nucleus and its release upon IP3 addition. Patch... Tipo: Journal article Palavras-chave: Single channel; Cell nucleus; IP3 receptor; Muscle. Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300015 Patch-clamp detection of macromolecular translocation along nuclear pores BJMBR Bustamante,J.O.; Varanda,W.A.. The present paper reviews the application of patch-clamp principles to the detection and measurement of macromolecular translocation along the nuclear pores. We demonstrate that the tight-seal 'gigaseal' between the pipette tip and the nuclear membrane is possible in the presence of fully operational nuclear pores. We show that the ability to form a gigaseal in nucleus-attached configurations does not mean that only the activity of channels from the outer membrane of the nuclear envelope can be detected. Instead, we show that, in the presence of fully operational nuclear pores, it is likely that the large-conductance ion channel activity recorded derives from the nuclear pores. We conclude the technical section with the suggestion that the best way to... Tipo: Info:eu-repo/semantics/article Palavras-chave: Nuclear pores; Nuclear envelope; Cell nucleus; Nucleocytoplasmic transport; Macromolecular transport; Gene activity; Gene expression; Transcriptional control; Ion channels; Patch-clamp; Measurement. Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000300003 Registros recuperados: 2 Primeira ... 1 ... Última

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