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Application of Response Surface Methodology for Optimizing Process Parameters in the Production of Amylase by Aspergillus flavusNSH9 under Solid State Fermentation BABT
Mustafa,Siti Ratina; Husaini,Ahmad; Hipolito,Cirilo Nolasco; Hussain,Hasnain; Suhaili,Nurshikin; Roslan,Hairul Azman.
Amylase is recognized as one of the important commercial enzymes. This group of enzymes has the ability in hydrolyzing starch into smaller oligosacharides. The present work aimed to determine the optimum fermentation conditions for maximum production of crude amylase enzyme by Aspergillus flavus NSH9 employing response surface methodology (RSM).Central composite design (CCD) was applied to determine the optimal fermentation condition with respect to the four main process parameters such as temperature, initial moisture content, pH and the incubation period. Solid state fermentation (SSF) was performed using 5.0 g of sago hampas inoculated with 1x107sporesmL-1following the experimental design obtained using CCD and further optimized by RSM. The initial...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Solid state fermentation; Aspergillus flavusNSH9; Sago hampas; Central Composite Design; Response Surface Methodology.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100416
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Influence of NaNO3 concentration and incident light intensity on Nannochloropsis oculata lipid accumulation BABT
Gris,Lara Regina Soccol; Paim,Anderson de Campos; Farenzena,Marcelo; Trierweiler,Jorge Otávio.
This study aimed at evaluating the best conditions for lipid accumulation in the marine microalga Nannochloropsis oculata in an airlift photobioreactor. Experiments were carried out following a central composite design with the following variables: temperature (19 to 29°C), sodium nitrate concentration (NaNO3) in the culture medium (f/2) (25 to 125 mg.L-1) and incident light intensity (49 to 140 µE.m-2s-1). The maximum lipid production was 132.4 mg.L-1 under the following conditions: 27°C, NaNO3 concentration 105 mg.L-1 and 122 µE.m-2.s-1, which was 30% of dry weight of the biomass.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nannochloropsis oculata; Airlift Photobioreactor; Central Composite Design; Lipid.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400018
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Scale up of dextran production from a mutant of Pediococcus pentosaceus (SPAm) using optimized medium in a bioreactor BABT
Patel,Seema; Kothari,Damini; Shukla,Rishikesh; Das,Debasish; Goyal,Arun.
The mutant of Pediococcus pentosaceus (SPAm) produced earlier by UV-mutagenesis exhibiting higher dextransucrase activity as compared to wild-type was used. The generated mutant SPAm gave 12.2 mg/ml, a 20% higher dextran than wild-type. Response surface methodology was carried out for further enhancement of dextran production. To enhance dextran production by the mutant SPAm, Plackett-Burman Design and a 2² full factorial Central Composite Design was employed. After response optimization, the optimum concentration of sucrose and yeast extract was 5.115% (w/v) and 0.635% (w/v), respectively. The experimental values of dextran 36.0 mg/ml at flask level and 35.0 mg/ml at bioreactor level were in good agreement with the predicted value of 40.8 mg/ml. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pediococcus pentosaceus; Dextran; Plackett-Burman Design; Central Composite Design; Response Surface Methodology.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600007
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