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| HAKIME-SILVA,RICARDO A.; VELLOSA,JOSÉ C.R.; KHALIL,NAJEH M.; KHALIL,OMAR A.K.; BRUNETTI,IGUATEMY L.; OLIVEIRA,OLGA M.M.F.. |
| Mushrooms possess nutritional and medicinal properties that have long been used for human health preservation and that have been considered by researchers as possible sources of free radical scavengers. In this work, the antioxidant properties of water extracts from Agaricus blazei Murill, produced by maceration and decoction, are demonstrated in vitro. Resistance to oxidation is demonstrated through three mechanisms: i) inhibition of enzymatic oxidative process, with 100% inhibition of HRP (horseradish peroxidase) and MPO (myeloperoxidase); ii) inhibition of cellular oxidative stress, with 80% inhibition of the oxidative burst of polymorphonuclear neutrophils (PMNs); and iii) direct action over reactive species, with 62% and 87% suppression of HOCl and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Agaricus blazei Murrill; Antioxidant; Oxidant species; Chemiluminescence; Polymorphonuclear neutrophils. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000301073 |
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| Aluminum (Al3+) intoxication is thought to play a major role in the development of Alzheimer's disease and in certain pathologic manifestations arising from long-term hemodialysis. Although the metal does not present redox capacity, it can stimulate tissue lipid peroxidation in animal models. Furthermore, in vitro studies have revealed that the fluoroaluminate complex induces diacylglycerol formation, 43-kDa protein phosphorylation and aggregation. Based on these observations, we postulated that Al3+-induced blood platelet aggregation was mediated by lipid peroxidation. Using chemiluminescence (CL) of luminol as an index of total lipid peroxidation capacity, we established a correlation between lipid peroxidation capacity and platelet aggregation. Al3+... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Aluminum; Lipid peroxidation; Chemiluminescence; Platelet aggregation. |
| Ano: 1997 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000500005 |
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| Paino,I.M.M.; Ximenes,V.F.; Fonseca,L.M. da; Kanegae,M.P.P.; Khalil,N.M.; Brunetti,I.L.. |
| The release of reactive oxygen specie (ROS) by activated neutrophil is involved in both the antimicrobial and deleterious effects in chronic inflammation. The objective of the present investigation was to determine the effect of therapeutic plasma concentrations of non-steroidal anti-inflammatory drugs (NSAIDs) on the production of ROS by stimulated rat neutrophils. Diclofenac (3.6 µM), indomethacin (12 µM), naproxen (160 µM), piroxicam (13 µM), and tenoxicam (30 µM) were incubated at 37ºC in PBS (10 mM), pH 7.4, for 30 min with rat neutrophils (1 x 10(6) cells/ml) stimulated by phorbol-12-myristate-13-acetate (100 nM). The ROS production was measured by luminol and lucigenin-dependent chemiluminescence. Except for naproxen, NSAIDs reduced ROS production:... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Non-steroidal anti-inflammatory drugs; Neutrophils; Myeloperoxidase; Hypochlorous acid; Reactive oxygen species; Chemiluminescence. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400007 |
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| Lambert, Christian; Nicolas, Jean-louis; Bultel, Valérie. |
| Using a chemiluminescence (CL) test, it had been previously demonstrated that Vibrio pectenicida, which is pathogenic to Pecten maximus larvae, was able to inhibit completely the CL activity of P, maximus hemocytes and partially inhibit those of Crassostrea gigas, Conversely, a Vibrio sp, strain, S322, pathogenic to C,gigas larvae was more active in reducing the CL activity of oyster hemocytes than of scallop hemocytes, Using this same CL biotest, V, pectenicida and S322 cytoplasmic extracts were shown to reproduce CL inhibition while the cytoplasmic extract of a nonpathogenic strain (U1, Pseudoalteromonas) was without effect. Moreover, cytoplasmic extract as well as live V, pectenicida cells provoked, within a few hours, death of P. maximus hemocytes... |
| Tipo: Text |
Palavras-chave: Bivalves; Hemocyte; Toxin; Chemiluminescence; Crassostrea gigas; Pecten maximus; Vibrio pectenicida. |
| Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/publication-466.pdf |
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| Vong, Lilita; Laes, Agathe; Blain, Stéphane. |
| A new method for the non-specific determination of iron-porphyrin-like complexes in natural waters has been developed. It is based on the chemiluminescent oxidation of the luminol in the presence of dioxygen (O-2) at pH 13. The method has been implemented in a FIA manifold that allowed the direct injection of seawater. The limit of detection is 0.11 nM of equivalent hemin (Fe-protoporphyrin IX). Fe2+, Fe3+, H2O2, siderophore (deferoxamin mesylate), humic acid and phytic acid did not interfere when the), were present at the concentrations expected in seawater. Metal free porphyrin and Mg, Cu, Co porphyrin complexes did not induce a significant chemiluminescent signal. Poisoned unfiltered samples could be stored for several weeks before analyses. The new... |
| Tipo: Text |
Palavras-chave: Chemiluminescence; Complexation; Flow injection; Seawater; Porphyrin; Iron. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3298.pdf |
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