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Influence of the neural tube/notochord complex on MyoD expression and cellular proliferation in chicken embryos BJMBR
Alves,H.J.; Alvares,L.E.; Gabriel,J.E.; Coutinho,L.L..
Important advances have been made in understanding the genetic processes that control skeletal muscle formation. Studies conducted on quails detected a delay in the myogenic program of animals selected for high growth rates. These studies have led to the hypothesis that a delay in myogenesis would allow somitic cells to proliferate longer and consequently increase the number of embryonic myoblasts. To test this hypothesis, recently segmented somites and part of the unsegmented paraxial mesoderm were separated from the neural tube/notochord complex in HH12 chicken embryos. In situ hybridization and competitive RT-PCR revealed that MyoD transcripts, which are responsible for myoblast determination, were absent in somites separated from neural tube/notochord...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chicken development; MyoD; Myogenesis; In situ hybridization; BrdU; Cellular proliferation.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200005
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