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	Provedor de dados BABT (1) BJM (1) Autor Araújo,Nathália Kelly de (1) Assis,Cristiane Fernandes de (1) Macedo,Gorete Ribeiro de (1) Pagnoncelli,Maria Giovana Binder (1) Rodrigues,Sueli (1) Silva,Nayane Macêdo Portela da (1) Tang,Mingli (1) Wang,Li (1) Wang,Peng (1) Zhang,Pingping (1) Zhou,Wei (1) Mais... Palavra-chave Chitosanase production (2) Cell immobilization (1) Chitosanase (1) Crude enzymes and TLC (1) Gongronella sp. JG (1) Paenibacillus ehimensis (1) Sodium alginate (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2013 (1) 2010 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Chitosanase production by Paenibacillus ehimensis and its application for chitosan hydrolysis BABT Pagnoncelli,Maria Giovana Binder; Araújo,Nathália Kelly de; Silva,Nayane Macêdo Portela da; Assis,Cristiane Fernandes de; Rodrigues,Sueli; Macedo,Gorete Ribeiro de. The chitosanase production by Paenibacillus ehimensis was studied in submerged cultures and the chitosan hydrolysis was evaluated by using these enzymes without purification. The bacterium produced inducibles enzymes after 12 h of growth in a culture medium containing 0.2% (w/v) of soluble chitosan as carbon source. The enzyme production was strongly repressed by the presence of glucose. The production started as soon as the available sugars finished in the culture medium. The maximum level of chitosanase activity was 500 U.L-1 at 36°C after 36 h incubation. The crude enzyme was optimally active at pH 6.0 and 55°C and in these conditions, the enzyme presented good stability (6 days). The enzyme without purification was used to hydrolyze the chitosan which... Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitosanase; Paenibacillus ehimensis; Chitosanase production; Crude enzymes and TLC. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600023 Enhancement of chitosanase production by cell immobilization of Gongronella sp. JG BJM Zhang,Pingping; Zhou,Wei; Wang,Peng; Wang,Li; Tang,Mingli. Chitosanase production of Gongronella sp. JG cells immobilized in calcium alginate gel and polyurethane foam was compared with that of the free cells, there was a 60% increase in the enzyme yield (2429 U/L) compared to the highest yield obtained from free cells (1513 U/L). The optimal immobilization parameters (concentrations of sodium alginate, calcium chloride, bead inoculums, bead diameter, etc) for the enhanced production of chitosanase were determined as: sodium alginate 2% (w/v), 0.1 M calcium chloride, inoculum 10 mL beads to 100 mL production media and 2.7 mm bead diameter. Maximum chitosanase production was achieved with initial pH of 5.5 and temperature of 30 ºC. The alginate beads had well stability, retained 85% ability of enzyme production... Tipo: Info:eu-repo/semantics/article Palavras-chave: Chitosanase production; Gongronella sp. JG; Cell immobilization; Sodium alginate. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100029 Registros recuperados: 2 Primeira ... 1 ... Última

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