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| Aires, Tania; Marba, Nuria; Serrao, Ester A.; Duarte, Carlos M.; Arnaud-haond, Sophie. |
| Molecular analyses of bacteria associated with photosynthetic organisms are often confounded by coamplification of the chloroplastidial 16S rDNA with the targeted bacterial 16S rDNA. This major problem has hampered progress in the characterization of bacterial communities associated to photosynthetic organisms and has limited the full realization of the potential offered by the last generation of metagenomics approaches. A simple and inexpensive method is presented, based on ethanol and bleach treatments prior to extraction, to efficiently discard a great part of chloroplastidial DNA without affecting the characterization of bacterial communities through pyrosequencing. Its effectiveness for the description of bacterial lineages associated to the green... |
| Tipo: Text |
Palavras-chave: 16S; Chloroplasts; Elimination; Green algae; Metagenomics. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00076/18739/16464.pdf |
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| ORTIZ,Daniel; MORENO,Flavio; DÍEZ,Maria Claudia. |
| ABSTRACT In the Amazon region, agroforestry systems (AFSs) are recommended as a sustainable production alternative for local communities. A common component in Amazonian AFSs are tropical fruit trees, which can form the canopy or grow in the understory. In this study, we evaluated the effect of high radiation on photosynthesis, growth and seedling survival of four Amazonian fruit-tree species: Theobroma cacao, Eugenia stipitata, Inga edulis and Psidium guajava. Growth, chlorophyll fluorescence, gas exchange, and leaf pigments were measured in seedlings of each species grown for 12 months inside shade houses with low (8%), medium (30%) and high relative illumination (100%). Eugenia stipitata and T. cacao had the lowest acclimation capacity to high solar... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acclimation; Agroforestry systems; Carotenoids; Chloroplasts; Photosystem II. |
| Ano: 2021 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672021000100001 |
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