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	Provedor de dados Biol. Res. (1) Genet. Mol. Biol. (1) Autor Drets,Máximo E. (1) HOLTZ,MARLIS (1) PHI-VAN,LOC (1) Santiñaque,Federico F. (1) ZIMMERMANN,KATRIN (1) Palavra-chave Chromatin structure (2) GAS41 (1) Human chromosomes (1) Lysozyme (1) Microdensitometry (1) Origin of bidirectional replication (1) Promoter (1) T-banding (1) Telomere (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Journal article (1) Ano 2007 (2) País Brazil (1) Chile (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Non-random distribution of high density chromatin detected at opposite ends of T-banded human metaphase chromosomes Genet. Mol. Biol. Santiñaque,Federico F.; Drets,Máximo E.. Previous research using microdensitometric scanning and computer graphic image analysis showed that T-banded segments of human metaphase chromosomes usually exhibit an asymmetrical distribution of high density (HD) chromatin between sister chromatids. Here, we employed the same methods to analyze HD chromatin distribution at opposite ends of T-banded human lymphocyte chromosomes. This study revealed that in most chromosomes with an asymmetrical distribution of HD chromatin at both ends, the highest densities of each arm were located in opposite chromatids. The frequency of this configuration was 0.792 per chromosome, indicating that the highest chromatin densities of the terminal segments of T-banded human chromosomes were non-randomly distributed at... Tipo: Info:eu-repo/semantics/article Palavras-chave: Human chromosomes; T-banding; Chromatin structure; Telomere; Microdensitometry. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600004 The chromatin structure of the lysozyme GAS41 origin of DNA replication changes during the cell cycle Biol. Res. ZIMMERMANN,KATRIN; HOLTZ,MARLIS; PHI-VAN,LOC. We used a rapid and simple protocol using lysolecithin for mapping HS sites in vivo. The protocol is based on partial digestion with DNase I of exponentially growing cells following permeabilization by short treatment with lysolecithin. Using this protocol, we analyzed the chromatin structure of the region surrounding two overlapping elements, an origin of bidirectional DNA replication and the GAS41 promoter, in chicken myelomonocytic HD11 cells arrested in G0, G0 and S phases as well as at the G0/S border. The results show that the chromatin of this region became more nuclease sensitive when cells were arrested in G0 phase and that this change in chromatin structure was reversible after the cells began to enter S phase Tipo: Journal article Palavras-chave: Chromatin structure; Origin of bidirectional replication; Lysozyme; GAS41; Promoter. Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200009 Registros recuperados: 2 Primeira ... 1 ... Última

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