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| Registros recuperados: 35 | |
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| Velázquez González, Julio César. |
| Dado que el picudo del chile es el principal problema fitosanitario en el cultivo de Capsicum spp., y las medidas de control no han logrado mitigar de forma eficiente las poblaciones de este insecto, se estudiaron los siguientes objetivos con el fin de conocer las interacciones planta insecto. Primero se valido un método de recolecta de volátiles, el cual demostró ser eficiente bajo las diferentes variables analíticas estudiadas, con % de recobro de 90-100 y DER menores a 10%. En segundo lugar se evaluó el potencial atrayente de estructuras de la planta como flores, yemas, frutos en desarrollo y frutos comerciales, encontrando que machos prefieren en primer lugar flores y después yemas, y las hembras en su primera opción eligieron yemas y después flores.... |
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Palavras-chave: Validación; Feromona; Capsicum; Sensilla; Cromatografía; Validation; Pheromone; Chromatography; Entomología y Acarología; Doctorado. |
| Ano: 2011 |
URL: http://hdl.handle.net/10521/557 |
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| Velázquez González, Julio César. |
| Dado que el picudo del chile es el principal problema fitosanitario en el cultivo de Capsicum spp., y las medidas de control no han logrado mitigar de forma eficiente las poblaciones de este insecto, se estudiaron los siguientes objetivos con el fin de conocer las interacciones planta insecto. Primero se valido un método de recolecta de volátiles, el cual demostró ser eficiente bajo las diferentes variables analíticas estudiadas, con % de recobro de 90-100 y DER menores a 10%. En segundo lugar se evaluó el potencial atrayente de estructuras de la planta como flores, yemas, frutos en desarrollo y frutos comerciales, encontrando que machos prefieren en primer lugar flores y después yemas, y las hembras en su primera opción eligieron yemas y después flores.... |
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Palavras-chave: Validación; Feromona; Capsicum; Sensilla; Cromatografía; Validation; Pheromone; Chromatography; Entomología y Acarología; Doctorado. |
| Ano: 2011 |
URL: http://hdl.handle.net/10521/557 |
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| Hsiao,Nai-Wan; Chen,Yeh; Kuan,Yi-Chia; Lee,Yen-Chung; Lee,Shuo-Kang; Chan,Hsin-Hua; Kao,Chao-Hung. |
| Background Aspartic proteases are a subfamily of endopeptidases that are useful in a variety of applications, especially in the food processing industry. Here we describe a novel aspartic protease that was purified from Peptidase R, a commercial protease preparation derived from Rhizopus oryzae. Results An aspartic protease sourced from Peptidase R was purified to homogeneity by anion exchange chromatography followed by polishing with a hydrophobic interaction chromatography column, resulting in a 3.4-fold increase in specific activity (57.5 × 10³ U/mg) and 58.8% recovery. The estimated molecular weight of the purified enzyme was 39 kDa. The N-terminal sequence of the purified protein exhibited 63-75% identity to rhizopuspepsins from various Rhizopus... |
| Tipo: Journal article |
Palavras-chave: Chromatography; Endopeptidase; Food processing industry; Homogeneity; Rhizopuspepsin. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000200006 |
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| QUEIROZ, S. C. do N. de; SOUZA, D. R. C. de; CERDEIRA, A. L.; PARAIBA, L. C.. |
| O cultivo de soja utiliza diversos tipos de fungicidas para controlar fungos fitopatogênicos, que, dependendo das características físico-químicas das moléculas, podem se acumular nos grãos e causar riscos à saúde humana. Desse modo, para verificar a presença de resíduos destas substâncias nesta matriz alimentar há necessidade de desenvolver e validar métodos analíticos que sejam sensíveis e confiáveis. Assim, o objetivo deste trabalho foi adaptar e validar um método para a determinação dos fungicidas não-iônicos ciproconazol, epoxiconazol, piraclostrobina e azoxistrobina em grãos de soja, para ser aplicado na validação de um modelo matemático cinético de bioconcentração, a fim de prever o acúmulo desses agrotóxicos nesse alimento. O método analítico... |
| Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) |
Palavras-chave: Mass spectroscopy; Soybean; Fungicida; Resíduo químico; Análise química; Soja; Cromatografia gasosa; Fungicides; Chemical residues; Chemical analysis; Chromatography. |
| Ano: 2017 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1090496 |
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| Bustamante, Paco; Bertrand, M; Boucaud Camou, E; Miramand, Pierre. |
| The subcellular fractionation of the digestive gland cells of the common cuttlefish Sepia officinalis was performed to investigate the distribution of metals between organelles and cytosol and the different cytosolic fractions separated by gel-filtration chromatography. Total metal concentrations vary over 3 orders of magnitude, ranging from dwt for Pb to dwt for Zn. With the exception of Cd, Co, and Cu, metals were mostly bound to the organelles. Whereas no specific organelle compartment was found for Mn, Ph, and Zn, Fe was mainly associated with nucleus, brown body, and "boule" fraction (i.e., 52%) and 44% of the total Ag was contained in the lysosomal and mitochondria enriched fraction. The link of metals with hydrosoluble proteins in the cytosolic... |
| Tipo: Text |
Palavras-chave: Sepia officinalis; Chromatography; Metallothionein; Detoxification; Trace element; Metal; Cephalopod. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-2532.pdf |
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| Menaldo,Danilo L.; Jacob-Ferreira,Anna L.; Bernardes,Carolina P.; Cintra,Adélia C. O.; Sampaio,Suely V.. |
| Background Snake venoms are complex mixtures of inorganic and organic components, mainly proteins and peptides. Standardization of methods for isolating bioactive molecules from snake venoms is extremely difficult due to the complex and highly variable composition of venoms, which can be influenced by factors such as age and geographic location of the specimen. Therefore, this study aimed to standardize a simple purification methodology for obtaining a P-I class metalloprotease (MP) and an acidic phospholipase A2 (PLA 2 ) from Bothrops atroxvenom, and biochemically characterize these molecules to enable future functional studies.Methods To obtain the toxins of interest, a method has been standardized using consecutive isolation steps. The purity level of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Snake venoms; Bothrops atrox; Toxins; Metalloprotease; Phospholipase A2; Isolation; Characterization; Chromatography. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100337 |
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| Shirmardi,SP; Shamsaei,M; Gandomkar,M; Saniei,E; Ghannadi,M; Zare,A. |
| Iranian scorpions belong mainly to the Buthidae and Scorpionidae families, distributed into 16 genera and 25 species. In Iran, similar to other parts of the world, there are a few known species of scorpions responsible for severe envenoming; amongst which Mesobuthus eupeus is the most common. Its venom contains several toxin fractions that may affect the ion channel. In the present study purification, labeling and biological evaluation of M. eupeus venom are described. For separation, soluble venom was loaded on a chromatography column packed with Sephadex G-50 gel. Subsequently, the fractions were collected according to UV absorption at a wavelength of 280 nm. Toxic fraction (F3) was loaded on an anionic ion exchanger resin and then on a cationic resin.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mesobuthus eupeus; Venom; Purification; Chromatography; Radiolabeling; Biodistribution. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400015 |
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| Salmanizadeh,Hossein; Babaie,Mahdi; Zolfagharian,Hossein. |
| Background The venom of the family Viperidae, including the saw-scaled viper, is rich in serine proteinases and metalloproteinases, which affect the nervous system, complementary system, blood coagulation, platelet aggregation and blood pressure. One of the most prominent effects of the snake venom of Echis carinatus (Ec) is its coagulation activity, used for killing prey. Materials and methods Subfractions F1A and F1B were isolated from Ec crude venom by a combination of gel chromatography (Sephadex G-75) and ion exchange chromatography on a DEAE-Sepharose (DE-52). These subfractions were then intravenously (IV) injected into NIH male mice. Blood samples were taken before and after the administration of these subfractions. Times for prothrombin, partial... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Snake venom; Procoagulant activity; Blood coagulation; Echis carinatus; Chromatography; LD50. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100302 |
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| Ghorbanpur,M; Zare Mirakabadi,A; Zokaee,F; Zolfagarrian,H; Rabiei,H. |
| Agkistrodon halys is one of several dangerous snake species in Iran. Among the most important signs and symptoms in patients envenomated by this snake is disseminated intravascular coagulation. A thrombin-like enzyme, called AH143, was isolated from Agkistrodon halys venom by gel filtration on a Sephadex G-50 column, ion-exchange chromatography on a DEAE-Sepharose and high performance liquid chromatography (HPLC) on a C18 column. In the final stage of purification, 0.82 mg of purified enzyme was obtained from 182.5 mg of venom. The purified enzyme showed a single protein band by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), under reducing conditions, and its molecular mass was found to be about 30 kDa. AH143 revealed clotting... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Iranian snake; Venom; Agkistrodon halys; Serine protease; Chromatography; Coagulant activity. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300005 |
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| Bordon,Karla C. F.; Wiezel,Gisele A.; Cabral,Hamilton; Arantes,Eliane C.. |
| BackgroundCrotalus durissus terrificus venom (CdtV) is one of the most studied snake venoms in Brazil. Despite presenting several well known proteins, its L-amino acid oxidase (LAAO) has not been studied previously. This study aimed to isolate, characterize and evaluate the enzyme stability of bordonein-L, an LAAO from CdtV.Methods The enzyme was isolated through cation exchange, gel filtration and affinity chromatography, followed by a reversed-phase fast protein liquid chromatography to confirm its purity. Subsequently, its N-terminal amino acid sequence was determined by Edman degradation. The enzyme activity and stability were evaluated by a microplate colorimetric assay and the molecular mass was estimated by SDS-PAGE using periodic acid-Schiff... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Crotalus durissus terrificus; L-amino acid oxidase; Rattlesnake; Enzyme activity; Enzyme stability; Chromatography; Snake venom; Yellow venom; Stabilization. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100335 |
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| TEIXEIRA,M.F.F.; SILVA,A.A.; NASCIMENTO,M.A.; VIEIRA,L.S.; TEIXEIRA,T.P.M; SOUZA,M.F.. |
| ABSTRACT: Tebuthiuron is one of the most widely used herbicides in the sugarcane culture and its characteristic is the long persistence in soil. When used without knowing its interactions with soil attributes, it can reduce the sustainability of cropping systems and contaminate surface and groundwaters. In this research, by using a high-performance liquid chromatography, the effects of adding organic matter in a Red-Yellow Latosol were evaluated, as for the sorption and desorption of tebuthiuron. It was concluded that there is a direct relation between the sorption of tebuthiuron and the organic matter content in Red-Yellow Latosols and there is an inverse relation for desorption. The hysteresis index was lower in samples with high organic matter content. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chromatography; Hysteresis index; Persistence. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582018000100283 |
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| Registros recuperados: 35 | |
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