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Interindividual size heteromorphism of NOR and chromosomal location of 5S rRNA genes in Iheringichthys labrosus BABT
Carvalho,Rafael Augusto de; Dias,Ana Lúcia.
Twenty-five specimens of Iheringichthys labrosus from the Capivara Reservoir were analysed cytogenetically. AgNORs were detected in a pair of ST chromosomes, in the telomeric region of the long arm. Some individuals showed size heteromorphism of this region between homologous chromosomes. Treatment with CMA3 displayed GC-rich regions corresponding to the AgNOR pair, plus other fluorescent staining. In situ hybridization by fluorescence (FISH) with the 18S rDNA probe showed only one pair of stained chromosomes, confirming the heteromorphism observed with AgNO3 and CMA3 in some individuals. The 5S rDNA probe revealed telomeric staining on the long arm of a pair of chromosomes of the ST-A group, probably different from the NOR pair.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish chromosome; AgNOR; In situ hibridization; Chromomycin A3; Heteromorphism.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100017
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Cytogenetic data on Astyanax jacuhiensis (Characidae) in the lago Guaíba and tributaries, Brazil Neotropical Ichthyology
Pacheco,Rosiley B.; Giuliano-Caetano,Lucia; Julio Junior,Horácio F.; Dias,Ana L..
Cytogenetic analyses were performed in Astyanax jacuhiensis from lago Guaíba, Brazil. The diploid number was 50, with a karyotype composed of 8m+30sm+4st+8a chromosomes, FN = 92. The AgNORs were observed in 2 to 5 chromosomes, with intra- and interindividual variation. The sm pair 8 observed always carried NORs on the short arms, presenting size heteromorphism between homologous. Fluorescence in situ hybridization (FISH) with an 18S rDNA probe only confirmed the location of ribosomal cistrons in the sm pair 8, and heteromorphism of these regions between the homologous chromosomes. C-banding revealed the occurrence of weak C-positive heterochromatin in the pericentromeric regions of several chromosomes, in addition to more evident bands interstitially...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chromosomes; Chromomycin A3; 18S rDNA; NOR.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252010000300013
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