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	Provedor de dados BABT (1) Genet. Mol. Biol. (1) Autor Felitti,Silvina (1) Li,Yaning (1) Liu,Daqun (1) Martínez,Eric J. (1) Ortiz,Juan Pablo A. (1) Pessino,Silvina C. (1) Podio,Maricel (1) Quarin,Camilo L. (1) Rodríguez,María P. (1) Siena,Lorena A. (1) Stein,Juliana (1) Yan,Hongfei (1) Yang,Wenxiang (1) Zhang,Yan (1) Mais... Palavra-chave Chromosome walking (2) Apomixis (1) Gene mapping (1) Molecular markers (1) Nonspecific primer anchored PCR (1) Random primer (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2012 (1) 2011 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última A nonspecific primer anchored PCR technique for chromosome walking BABT Zhang,Yan; Liu,Daqun; Yang,Wenxiang; Li,Yaning; Yan,Hongfei. A chromosome walking method was improved in this work. The new method was named as nonspecific primer anchored PCR (NPA-PCR). Nested gene specific primers were designed based on the known region and long random primer using degeneracy oligonucleotides for nonspecific anchoring. Annealing temperatures were varied to control the priming. Target sequences were obtained by PCR with random primer and gene-specific primer. Nonspecific sequence with long random primers at both ends formed stem loop structure due to inverted terminal repeats. The method was employed to isolate a gene with newly-isolated actinomycin producing strain Streptomyces setonii Z-L-22. A 0.77 kb fragment of actinomycin synthetase gene cluster was isolated from the strain. The fragments of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Nonspecific primer anchored PCR; Chromosome walking; Random primer. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100013 Sequence characterization, in silico mapping and cytosine methylation analysis of markers linked to apospory in Paspalum notatum Genet. Mol. Biol. Podio,Maricel; Rodríguez,María P.; Felitti,Silvina; Stein,Juliana; Martínez,Eric J.; Siena,Lorena A.; Quarin,Camilo L.; Pessino,Silvina C.; Ortiz,Juan Pablo A.. In previous studies we reported the identification of several AFLP, RAPD and RFLP molecular markers linked to apospory in Paspalum notatum. The objective of this work was to sequence these markers, obtain their flanking regions by chromosome walking and perform an in silico mapping analysis in rice and maize. The methylation status of two apospory-related sequences was also assessed using methylation-sensitive RFLP experiments. Fourteen molecular markers were analyzed and several protein-coding sequences were identified. Copy number estimates and RFLP linkage analysis showed that the sequence PnMAI3 displayed 2-4 copies per genome and linkage to apospory. Extension of this marker by chromosome walking revealed an additional protein-coding sequence mapping... Tipo: Info:eu-repo/semantics/article Palavras-chave: Apomixis; Chromosome walking; Gene mapping; Molecular markers. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500018 Registros recuperados: 2 Primeira ... 1 ... Última

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