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	Provedor de dados BJPS (1) Genet. Mol. Biol. (1) Autor Fan,Alex Xiu Cheng (1) Hahn,Sinuhe (1) Hirata,Rosario Dominguez Crespo (1) Holzgreve,Wolfgang (1) Kohler,Corina (1) Oliveira,Isadora Bernardo David de (1) Radpour,Ramin (1) Xia,Peng (1) Zachariah,Rebecca (1) Zhong,Xiao Yan (1) Mais... Palavra-chave Circulating cell-free DNA (2) Biomarkers (1) CcfDNA (1) Colorectal Neoplasms/diagnosis (1) Colorectal cancer (1) Diagnosis (1) Mitochondrial DNA (1) Neoplastic Cells/circulating. (1) Nuclear DNA (1) Prognosis (1) Quantitative PCR (1) Real-time PCR (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2018 (1) 2009 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Simultaneous quantitative assessment of circulating cell-free mitochondrial and nuclear DNA by multiplex real-time PCR Genet. Mol. Biol. Xia,Peng; Radpour,Ramin; Zachariah,Rebecca; Fan,Alex Xiu Cheng; Kohler,Corina; Hahn,Sinuhe; Holzgreve,Wolfgang; Zhong,Xiao Yan. Quantification of circulating nucleic acids in plasma and serum could be used as a non-invasive diagnostic tool for monitoring a wide variety of diseases and conditions. We describe here a rapid, simple and accurate multiplex real-time PCR method for direct synchronized analysis of circulating cell-free (ccf) mitochondrial (mtDNA) and nuclear (nDNA) DNA in plasma and serum samples. The method is based on one-step multiplex real-time PCR using a FAM-labeled MGB probe and primers to amplify the mtDNA sequence of the ATP 8 gene, and a VIC-labeled MGB probe and primers to amplify the nDNA sequence of the glycerinaldehyde-3-phosphate-dehydrogenase (GAPDH) gene, in plasma and serum samples simultaneously. The efficiencies of the multiplex assays were measured in... Tipo: Info:eu-repo/semantics/article Palavras-chave: Circulating cell-free DNA; Mitochondrial DNA; Nuclear DNA; Real-time PCR; Quantitative PCR. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100003 Circulating cell-free DNA as a biomarker in the diagnosis and prognosis of colorectal cancer BJPS Oliveira,Isadora Bernardo David de; Hirata,Rosario Dominguez Crespo. Abstract Colorectal cancer (CRC) is a disease without evident clinical symptoms in early stages, leading to late diagnosis and disease management. Current diagnostic and prognostic tools require invasive procedures and circulating molecular biomarkers fail to have optimal sensitivity and specificity. Circulating biomarkers with high clinical performance may be valuable for early diagnosis and prognosis of CRC. The purpose of this review was to investigate the application of circulating cell-free DNA (ccfDNA) in CRC diagnosis and prognosis and the analytical methods used in blood samples in articles published between 2005 and 2016. Based on specific inclusion and exclusion criteria, 26 articles were selected. Most studies used ccfDNA quantification as the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Colorectal Neoplasms/diagnosis; Colorectal cancer; Circulating cell-free DNA; CcfDNA; Prognosis; Diagnosis; Biomarkers; Neoplastic Cells/circulating.. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000100406 Registros recuperados: 2 Primeira ... 1 ... Última

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