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	Provedor de dados 52 (1) Autor Khudyakov,Andrey Nikolaevich (1) Polezhaeva,Tatyana Vitalyevna (1) Sergushkina,Marta Igorevna (1) Solomina,Olga Nurzadinovna (1) Zaitseva,Oksana Olegovna (1) Palavra-chave Clathrates (1) Cryopreservation (1) Krypton (1) Leukocytes (1) Xenon (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2019 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Use of Inert Gases for the Preservation of Nuclear Blood Cells 52 Khudyakov,Andrey Nikolaevich; Polezhaeva,Tatyana Vitalyevna; Zaitseva,Oksana Olegovna; Sergushkina,Marta Igorevna; Solomina,Olga Nurzadinovna. Abstract The subject of the study was the stability of human white blood cell membranes subject to noble gases (xenon ad krypton, 0.6 mPa) clathrate cryoanabiosis (‒80°C). A unique portable stainless steel low pressure container with a compartment for flexible plastic container was designed to ensure that the cells are saturated with gases. The samples were warmed after 1 and 30 days in a water bath (+38°C) for 35-50 sec, while the container was being tilted (2-3 times per second), until the temperature of the biological object reached +3±1°C. It was demonstrated that after 30 days of clathrate anabiosis (-80°C) over 95% (of the original number) of leukocytes remain viable, and cell membranes of 54.5±3.4% of them is resistant to trypan blue; granulocyte... Tipo: Info:eu-repo/semantics/article Palavras-chave: Clathrates; Cryopreservation; Xenon; Krypton; Leukocytes. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100404 Registros recuperados: 1 Primeira ... 1 ... Última

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