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Effect of amikacin, cephalothin, clindamycin and vancomycin on in vitro fibroblast growth Genet. Mol. Biol.
Souza,Fernanda Timm Seabra; Mello,Alexandre Silva de; Michelin,Kristiane; Coelho,Janice Carneiro.
The effect of four antibiotics (amikacin, clindamycin, cephalothin and vancomycin) was investigated considering that bacterial infection in fibroblasts cultures is a very frequent event. The investigation included the effect of the antibiotics on fibroblast growth and on the activity of the enzyme glucocerebrosidase. The antibiotics were added to the fibroblast cultures and cell growth was evaluated by counting the number of cells and their viability. After cell harvesting, the enzyme activity and content of protein were measured. The results allowed us to conclude that none of the antibiotics affected the cellular number nor the cellular viability. The content of protein decreased when cephalothin and clindamycin were added to the cultures, and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amikacin; Cephalothin; Clindamycin; Vancomycin; Fibroblasts; Cell culture.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000300023
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Use of the D test method to detect inducible clindamycin resistance in coagulase negative staphylococci (CoNS) BJID
Perez,Leandro Reus Rodrigues; Caierão,Juliana; Antunes,Ana Lúcia Souza; d'Azevedo,Pedro Alves.
According to the National Committee for Clinical Laboratory Standards (NCCLS, 2004), a method to evaluate the inducible clindamycin resistance in accordance with an approach of the disks of erythromycin and clindamycin - the D test - has been reported. We analyzed the performance of this method in 200 coagulase negative staphylococci (CoNS) strains obtained from blood cultures of hospitalized patients at a general hospital in Southern Brazil. Twenty-seven clinical isolates with suitable profile (erythromycin-resistant and clindamycin-susceptible) were evaluated for the D test realization. Thus, only 5 CoNS were D test positive. The D test method showed to be simple and an important technique in the detection of inducible clindamycin resistance.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Clindamycin; Resistance; D test.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000200002
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Phenotypic and molecular characterization of resistance to macrolides, lincosamides and type B streptogramin of clinical isolates of Staphylococcus spp. of a university hospital in Recife, Pernambuco, Brazil BJID
Pereira,Jussyêgles Niedja da Paz; Rabelo,Marcelle Aquino; Lima,Jailton Lobo da Costa; Neto,Armando Monteiro Bezerra; Lopes,Ana Catarina de Souza; Maciel,Maria Amélia Vieira.
Abstract Introduction There is a mechanism of macrolide resistance in Staphylococcus spp. which also affects the lincosamides and type B streptogramins characterizing the so-called MLSB resistance, whose expression can be constitutive (cMLSB) or inducible (iMLSB) and is encoded mainly by ermA and ermC genes. The cMLSB resistance is easily detected by susceptibility testing used in the laboratory routine, but iMLSB resistance is not. Therapy with clindamycin in cases of infection with isolated iMLSB resistance may fail. Objective To characterize the phenotypic (occurrence of cMLSB and iMLSB phenotypes) and molecular (occurrence of ermA and ermC genes) profiles of MLSB resistance of clinical isolates of susceptible and methicillin-resistant Staphylococcus...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus; Clindamycin; Inducible resistance; Erm genes.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000300010
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Success stories about severe pneumonia caused by Panton-Valentine leucocidin-producing Staphylococcus aureus BJID
Elisabeth,Paramythiotou; Maria,Souli; Irene,Galani; Helen,Giamarellou; Apostolos,Armaganidis.
We describe three cases of community-acquired necrotizing pneumonia which were caused by Panton-Valentine leucocidin-producing strains of Staphylococcus aureus (one of them methicillin sensitive). All cases were successfully treated without any sequelae for the patients due to the prompt initiation of adequate antimicrobial therapy. High suspicion toward this fatal pathogen was the key to the successful outcome of the patients.
Tipo: Info:eu-repo/semantics/report Palavras-chave: Community-acquired pneumonia; Clindamycin; Staphylococcus aureus; Panton-Valentine leukocidin.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000300341
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Evaluation of culture media for selective enumeration of bifidobacteria and lactic acid bacteria BJM
Süle,Judit; Kõrösi,Tímea; Hucker,Attila; Varga,László.
The purpose of this study was to test the suitability of Transgalactosylated oligosaccharides-mupirocin lithium salt (TOS-MUP) and MRS-clindamycin-ciprofloxacin (MRS-CC) agars, along with several other culture media, for selectively enumerating bifidobacteria and lactic acid bacteria (LAB) species commonly used to make fermented milks. Pure culture suspensions of a total of 13 dairy bacteria strains, belonging to eight species and five genera, were tested for growth capability under various incubation conditions. TOS-MUP agar was successfully used for the selective enumeration of both Bifidobacterium animalis subsp. lactis BB-12 and B. breve M-16 V. MRS-CC agar showed relatively good selectivity for Lactobacillus acidophilus, however, it also promoted the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mupirocin; Clindamycin; Ciprofloxacin; Bifidobacterium; Lactobacillus acidophilus.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300035
Registros recuperados: 5
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