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Genome analysis of thirteen Colombian clostridial strains by pulsed field gel electrophoresis Electron. J. Biotechnol.
Quilaguy Ayure,Diana Milena; Suárez Moreno,Zulma Rocío; Aristizábal Gutierrez,Fabio Ancizar; Bernal Morales,Jose Mauricio; Montoya Castaño,Dolly.
Pulsed field gel electrophoresis was used for estimating the size of the genome and evaluating the presence of megaplasmids in 13 native Colombian solventogenic Clostridium strains. DNA preparation and purification were optimised for obtaining differentiated restriction fragments in electrophoresis. Genomic DNA was digested with ApaI, Eco52I, SmaI and XhoI enzymes. Estimated genome size for native strains ranged from 4.0 to 4.2 mega base pairs. Larger sized plasmids were detected and the presence of genes related to megaplasmid pSOL1 was determined by polymerase chain reaction. adc gene region amplification suggested that genes related to solventogenesis in native strains may be located in an extra-chromosomal element. Determining genome size provides...
Tipo: Journal article Palavras-chave: Clostridium; Genome size; PFGE; PSOL1.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500009
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Genome sequence of the H2-producing Clostridium beijerinckii strain Br21 isolated from a sugarcane vinasse treatment plant Genet. Mol. Biol.
Fonseca,Bruna Constante; Riaño-Pachón,Diego Mauricio; Guazzaroni,María-Eugenia; Reginatto,Valeria.
Abstract We report on the nearly complete genome sequence of Clostridium beijerinckii strain Br21, formerly isolated from a sugarcarne vinasse wastewater treatment plant. The resulting genome is ca. 5.9 Mbp in length and resembles the size of previously published C. beijerinckii genomes. We annotated the genome sequence and predicted a total of 5323 genes. Strain Br21 has a genetic toolkit that allows it to exploit diverse sugars that are often found after lignocellulosic biomass pretreatment to yield products of commercial interest. Besides the whole set of genes encoding for enzymes underlying hydrogen production, the genome of the new strain includes genes that enable carbon sources conversion into butanol, ethanol, acetic acid, butyric acid, and the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Clostridium; Biofuels; Biohydrogen; Beta-glucosidase.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100139
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Microbiological profile and aerobic stability of Tifton 85 bermudagrass silage with different additives R. Bras. Zootec.
Neres,Marcela Abbado; Zambom,Maximiliane Alavarse; Fernandes,Tatiane; Castagnara,Deise Dalazen; Rodrigues,Juliana Ferraz Huback; Taffarel,Loreno Egidio; Javorski,Cleovani Rossi; Pozza,Magali Soares dos Santos.
The objective of this study was to evaluate the microbiological profile and aerobic stability of silage with Tifton 85 bermudagrass and different additives and wilting. The studied treatments were: pre-drying in the sun for two hours before ensiling; use of bacterial-enzymatic inoculant; addition of soybean peel; addition of corn grits and use of salt in the surface layer of the silo. The experimental design was completely randomized with six treatments and four replications. Plants of Tifton 85 bermudagrass with 38 days were ensiled in experimental silos with Bunsen valves, with compacting density of 236 kg silage per m³ for Tifton 85 bermudagrass, which was pre-dried in the sun; the average silage was 294 kg m-3 for the other treatments. The ratios...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Clostridium; Enterobacteria; PH silage; Temperature silage; Yeasts.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982013000600001
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Isolation and characterization of cultivable fermentative bacteria from the intestine of two edible snails, Helixpomatia and Cornu aspersum (Gastropoda: Pulmonata) Biol. Res.
CHARRIER,MAR YVONNE; FONTY,GERARD; GAILLARD-MARTINIE,BRIGITTE; AINOUCHE,KADER; ANDANT,GERARD.
The intestinal microbiota of the edible snails Cornu aspersum fSyn: H. aspersa), and Helix pomatia were investigated by culture-based methods, 16S rRNA sequence analyses and phenotypic characterisations. The study was carried out on aestivating snails and two populations of H. pomatia were considered. The cultivable bacteria dominated in the distal part of the intestine, with up to 5.10(9) CFU g -1, but the Swedish H. pomatia appeared significantly less colonised, suggesting a higher sensitivity of its microbiota to climatic change. All the strains, but one, shared ≥ 97% sequence identity with reference strains. They were arranged into two taxa: the Gamma Proteobacteria with Buttiauxella, Citrobacter, Enterobacter, Kluyvera, Obesumbacterium,...
Tipo: Journal article Palavras-chave: Clostridium; Edible snails; Enterobacteriaceae; Enterococci; Gut microbiota; Lactococci.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500010
Registros recuperados: 4
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