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| Ziech,Rosangela Estel; Farias,Luana D’Avila; Ardisson-Araújo,Daniel Mendes Pereira; Camargo,Gregório Miguel Ferreira de; Frey,Joachim; Vargas,Agueda Castagna de. |
| ABSTRACT: Clostridium chauvoei toxin A (CctA), neuraminidase (NanA), and flagellin (FliC) proteins contribute to the pathogenicity of Clostridium chauvoei, the causative agent of blackleg in cattle. The aim of this study was to analyze the genetic variability of cctA, nanA, and fliC genes in C. chauvoei isolates from the Rio Grande do Sul and São Paulo state- Brazil, during different sampling periods. The presence of these genes was verified through PCR amplification and partial gene sequencing of 17 strains. Alignment of PCR amplicons combined with bioinformatics analysis was used in an attempt to study the variability across C. chauvoei solates. The similarity among the partial sequences of cctA and nanA genes was 100%. The sequencing of fliC revealed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Blackleg; Clostridium chauvoei toxin A; Neuraminidase; Flagellin. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000500452 |
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