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| Lechable, Marion; Jan, Anne-cecile; Duchene, Axel; Uveira, Julie; Weissbourd, Brandon; Gissat, Loann; Collet, Sophie; Gilletta, Laurent; Chevalier, Sandra; Leclère, Lucas; Peron, Sophie; Barreau, Carine; Lasbleiz, Régis; Houliston, Evelyn; Momose, Tsuyoshi. |
| The jellyfish species Clytia hemisphaerica (Cnidaria, Hydrozoa) has emerged as a new experimental model animal in the last decade. Favorable characteristics include a fully transparent body suitable for microscopy, daily gamete production and a relatively short life cycle. Furthermore, whole genome sequence assembly and efficient gene editing techniques using CRISPR/Cas9 have opened new possibilities for genetic studies. The quasi-immortal vegetatively-growing polyp colony stage provides a practical means to maintain mutant strains. In the context of developing Clytia as a genetic model, we report here an improved whole life cycle culture method including an aquarium tank system designed for culture of the tiny jellyfish form. We have compared different... |
| Tipo: Text |
Palavras-chave: Animal culture; Cnidarian; Developmental biology; Genetics; Jellyfish. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00656/76820/77996.pdf |
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| Lazcano-Pérez,Fernando; Zavala-Moreno,Ariana; Rufino-González,Yadira; Ponce-Macotela,Martha; García-Arredondo,Alejandro; Cuevas-Cruz,Miguel; Gómez-Manzo,Saúl; Marcial-Quino,Jaime; Arreguín-Lozano,Barbarín; Arreguín-Espinosa,Roberto. |
| Abstract Background Cnidarian venoms and extracts have shown a broad variety of biological activities including cytotoxic, antibacterial and antitumoral effects. Most of these studied extracts were obtained from sea anemones or jellyfish. The present study aimed to determine the toxic activity and assess the antitumor and antiparasitic potential of Palythoa caribaeorum venom by evaluating its in vitro toxicity on several models including human tumor cell lines and against the parasite Giardia intestinalis. Methods The presence of cytolysins and vasoconstrictor activity of P. caribaeorum venom were determined by hemolysis, PLA2 and isolated rat aortic ring assays, respectively. The cytotoxic effect was tested on HCT-15 (human colorectal adenocarcinoma),... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cnidarian; Palythoa caribaeorum; Cytotoxin; Antitumoral effect; Giardiasis. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100306 |
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