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| Zhao,Qing; Jing,Ji; Wang,Gang; Wang,Jie Hua; Feng,Yuan Yuan; Xing,Han Wen; Guan,Chun Feng. |
| Although Agrobacterium-mediated transformation protocols for many economically important plant species have been well established, protocol for a number of flowering plants including Anthurium andraeanum remains challenging. In this study, we report success in generating transgenic Anthurium andraeanum cv Arizona using Agrobacterium GV3101 strain harboring a binary vector carrying gfp as a reporter gene. The possibility of facilitating the screening process for transgenic plants expressing functional proteins using gfp marker was explored. In order to realize high transformation efficiency, different explant sources including undifferentiated callus pieces and petioles were compared for their regeneration efficiency and susceptibility to... |
| Tipo: Journal article |
Palavras-chave: Cocultivation; Gene transfer; Green fluorescent protein; Regeneration. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500009 |
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| Arcos-Ortega,Guadalupe Fabiola; Chan-Kuuk,Rafael Antonio; González-Kantún,Wilma Aracely; Souza-Perera,Ramón; Nakazawa-Ueji,Yumi Elena; Avilés-Berzunza,Elidé; Godoy-Hernández,Gregorio; Lawton,Michael A; Zúñiga Aguilar,José Juan. |
| Most of the pepper species of the genus Capsicum have been recalcitrant to efficient Agrobacterium tumefaciens-mediated stable or transient, genetic transformation. In the present work, we optimized a protocol for transient transformation of the Habanero pepper (Capsicum chinense Jacq.) through the standardization of several experimental factors. These included the age of the plants, the temperature, the length of co-cultivation, the application of a negative (vacuum) and/or a positive (infiltration) pressure, along with micro injection, the use of acetosyringone during the bacterial culturing, and modification of the pH during the GUS assay to eliminate the endogenous β-glucuronidase activity. The standardized protocol, which yielded nearly 55%... |
| Tipo: Journal article |
Palavras-chave: Cocultivation; GUS assay. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400007 |
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