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The combined analysis as the best strategy for Dual RNA-Seq mapping Genet. Mol. Biol.
Espindula,Eliandro; Sperb,Edilena Reis; Bach,Evelise; Passaglia,Luciane Maria Pereira.
Abstract In Dual RNA-Seq experiments the simultaneous extraction of RNA and analysis of gene expression data from both interacting organisms could be a challenge. One alternative is separating the reads during in silico data analysis. There are two main mapping methods used: sequential and combined. Here we present a combined approach in which the libraries were aligned to a concatenated genome to sort the reads before mapping them to the respective annotated genomes. A comparison of this method with the sequential analysis was performed. Two RNA-Seq libraries available in public databases consisting of a eukaryotic (Zea mays) and a prokaryotic (Herbaspirillum seropediceae) organisms were mixed to simulate a Dual RNA-Seq experiment. Libraries from real...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dual RNA-Seq; Sequential analysis; Combined analysis; Mapping strategies.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000500803
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Combination of lettuce and rocket cultivars in two cultures intercropped with carrots Horticultura Brasileira
Porto,Vânia CN; Bezerra Neto,Francisco; Lima,Jailma SS de; Barros Júnior,Aurélio P; Moreira,Joserlan N.
The intercropping practice is a viable technological option for the production of vegetables and the success of the association between crops will be greater the greater the complementarity between them. The present work was conducted from June to November 2006, at Universidade Federal Rural do Semi-Árido, Rio Grande do Norte State, Brazil, to evaluate the productive performance of two lettuce and two rocket cultivars in two cultures in strip-intercropping systems. The experimental design was randomized complete blocks, with five replications and treatments arranged in a 2 x 2 + 2 factorial. The treatments consisted of the combination of two lettuce cultivars (Babá de Verão and Tainá) and two of rocket (Cultivada and Folha Larga) intercropped with carrot...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lactuca sativa; Daucus carota; Eruca sativa; Agronomic efficiency; Combined analysis.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362011000300025
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