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Optimization of phenoxazinone synthase production by response surface methodology and its application in Congo red decolourization Electron. J. Biotechnol.
Jia,Honghua; Gao,Zhen; Ma,Yingying; Zhong,Chao; Xie,Yingchun; Zhou,Hua; Wei,Ping.
Background: Enzymatic decolourization has been recently proposed as a promising and eco-friendly method for treatment of synthetic dye-contaminated wastewaters. However, the processes require large quantities of enzymes, attracting significant attention in developing efficient methods for mass production of multifunctional enzymes. Several methods such as response surface methodology (RSM) and orthogonal experiment have been applied to optimize the parameters in bioprocesses for enzyme production. Results: In the present study, a laccase-like enzyme, phenoxazinone synthase (PHS) originated from Streptomyces antibioticus was recombinantly expressed in Escherichia coli BL21 (DE3). The production of PHS in E. coli BL21 was optimized by response surface...
Tipo: Journal article Palavras-chave: Congo red; Decolourization; Laccase; Phenoxazinone synthase; Response surface methodology.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000500002
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Azo Dye Mineralization by Phanerochaete Chysosporium in a Sequencing Bath Reactor BABT
Wanderley,Carlos Ronald Pessoa; Andrade,Marcus Vinícius; Pereira,Luciana José; Silva,Gloria Maria Marinho; Pessoa,Kelly Rodrigues.
ABSTRACT The mineralization of the azo dye congo red by the fungi Phanerochaete chrysosporium was studied in two sequential batch bioreactors (R1 and R2), operated in cycles of 48 h (step I) and 24 h (step II). In step I, glucose concentration was 1 g.L-1 in both reactors and in step II, 1 g.L-1 of glucose was maintained in R1, but R2 received no addition of glucose. In step I, the average dye removal efficiencies were 76 ± 29 % (R1) and 53 ± 15% (R2), while in step II the averages recorded for dye removal for R1 and R2 were 84 ± 15 and 70 ± 28%, respectively. The rates of dye removal were 0.04 h-1 in R1 and 0.03 h-1 in R2 in step I. Higher rates were obtained in step II, 0,07 h-1 and 0,02 h-1 for R1 and R2, respectively. The highest dye removal occurred...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biodegradation; Congo red; Phanerochaete chrysosporium; Sequential bioreactors.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100606
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Evaluation of sugar-cane bagasse as bioadsorbent in the textile wastewater treatment contaminated with carcinogenic congo red dye BABT
Raymundo,Aline Sartório; Zanarotto,Romina; Belisário,Marciela; Pereira,Madson de Godoi; Ribeiro,Joselito Nardy; Ribeiro,Araceli Verónica Flores Nardy.
A methodology involving sugar cane bagasse bioadsorbent was developed in order to remove the carcinogenic congo red dye from aqueous medium. The results showed high efficiency with retention of 64 ± 6% in synthetic congo red solution and 94 ± 5% in effluent enriched with congo red, at 10.0 g of the bioadsorbent. The adsorption system provided a maximum adsorption capacity of 4.43 mg/g. Tests showed independence adsorption properties, when compared with the column flow rates. The treatment units could be operated with flexibility. From the results, it was possible to conclude that sugar cane bagasse could be an adequate bioadsorbent.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sugar cane bagasse; Congo red; Bioadsorption.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400023
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Azo dye degradation by Phanerochaete chrysosporium in the medium enriched with nitrogen in the presence of primary co-substrate BABT
Andrade,Marcus Vinicius Freire; Silva,Karla Mayara Lima da; Siqueira,João Paulo da Silva; Wanderley,Carlos Ronald Pessoa; Araújo,Rinaldo dos Santos; Marinho,Gloria; Rodrigues,Kelly.
This work sought to evaluate the ability of fungi Phanerochaete chryosporium to degrade congo red azo dye in batch reactor, evaluate the influence of glucose and wheat bran as co-substrates on the removal of the dye in the medium and investigate the influence of ammonium chloride, ammonium nitrate and ammonium sulfate as the inorganic nitrogen source for the process. Wheat bran was not effective satisfactorily for the removal of dye and organic matter had no desired effect for the removal of color and organic matter and showed the lowest values of k2, 0.008 M-1.d-1and 0.0004 M-1.d-1, respectively. Glucose presented the best response with the highest final percentage of dye removal (97%) and rate of dye removal (0.017 M-1.d-1), without adding an external...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Wheat bran; Glucose; Congo red; Fungi; Nitrogen.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500019
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Mycoremediation of Congo red dye by filamentous fungi BJM
Bhattacharya,Sourav; Das,Arijit; G,Mangai.; K,Vignesh.; J,Sangeetha..
Azo, anthroquinone and triphenylmethane dyes are the major classes of synthetic colourants, which are difficult to degrade and have received considerable attention. Congo red, a diazo dye, is considered as a xenobiotic compound, and is recalcitrant to biodegradative processes. Nevertheless, during the last few years it has been demonstrated that several fungi, under certain environmental conditions, are able to transfer azo dyes to non toxic products using laccases. The aim of this work was to study the factors influencing mycoremediation of Congo red. Several basidiomycetes and deuteromycetes species were tested for the decolourisation of Congo red (0.05 g/l) in a semi synthetic broth at static and shaking conditions. Poor decolourisation was observed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Azo Dyes; Congo red; Laccases; Mycoreme.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400040
Registros recuperados: 5
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