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	Provedor de dados Anais da ABC (AABC) (1) Autor Carmona,Adriana K. (1) Juliano,Luiz (1) Juliano,Maria Aparecida (1) Palavra-chave Angiotensin I-converting enzyme (1) Continuous recording assay (1) FRET substrates (1) Fluorescence resonance energy transfer (1) Neprilisin (1) Proteolytic enzymes (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Ano 2009 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última The use of Fluorescence Resonance Energy Transfer (FRET) peptidesfor measurement of clinically important proteolytic enzymes Anais da ABC (AABC) Carmona,Adriana K.; Juliano,Maria Aparecida; Juliano,Luiz. Proteolytic enzymes have a fundamental role in many biological processes and are associated with multiple pathological conditions. Therefore, targeting these enzymes may be important for a better understanding of their function and development of therapeutic inhibitors. Fluorescence Resonance Energy Transfer (FRET) peptides are convenient tools for the study of peptidases specificity as they allow monitoring of the reaction on a continuous basis, providing a rapid method for the determination of enzymatic activity. Hydrolysis of a peptide bond between the donor/acceptor pair generates fluorescence that permits the measurement of the activity of nanomolar concentrations of the enzyme. The assays can be performed directly in a cuvette of the fluorimeter or... Tipo: Info:eu-repo/semantics/article Palavras-chave: Continuous recording assay; Fluorescence resonance energy transfer; FRET substrates; Proteolytic enzymes; Angiotensin I-converting enzyme; Neprilisin. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652009000300005 Registros recuperados: 1 Primeira ... 1 ... Última

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