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Differential expression of pathogenicity- and virulence-related genes of Xanthomonas axonopodis pv. citri under copper stress Genet. Mol. Biol.
Palmieri,Ana Carolina Basílio; Amaral,Alexandre Morais do; Homem,Rafael Augusto; Machado,Marcos Antonio.
In this study, we used real-time quantitative PCR (RT-qPCR) to evaluate the expression of 32 genes of Xanthomonas axonopodis pv. citri related to pathogenicity and virulence that are also involved in copper detoxification. Nearly all of the genes were up-regulated, including copA and copB. Two genes homologous to members of the type II secretion system (xcsH and xcsC) and two involved in the degradation of plant cell wall components (pglA and pel) were the most expressed in response to an elevated copper concentration. The type II secretion system (xcs operon) and a few homologues of proteins putatively secreted by this system showed enhanced expression when the bacteria were exposed to a high concentration of copper sulfate. The enhanced expression of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Copper resistance; Citrus canker; Real-time quantitative PCR.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200024
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