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Cellulase production by thermophilic Bacillus sp: SMIA-2 and its detergent compatibility Electron. J. Biotechnol.
Ladeira,Silvania A; Cruz,Erica; Delatorre,Andréia B; Barbosa,João B; Leal Martins,Meire Lelis.
Background This paper reports the production of cellulase by thermophilic Bacillus sp. SMIA-2 using sugarcane bagasse and corn steep liquor as substrates. Some biochemical properties of the enzyme were also assessed for the purposes of exploiting its potential in the detergent industry, as well as other suitable applications. Results Bacillus sp. produced cellulases when cultivated at 50°C in liquid cultures containing sugarcane bagasse and corn steep liquor. Maximum avicelase (0.83 U mL-1) and CMCase (0.29 U mL-1) activities were reached in 120 h and 168 h of culturing time, respectively. The avicelase and CMCase presented an optimum activity at pH of 7.5 and 8.0, respectively. The maximum stability of avicelase and CMCase was observed at a pH range...
Tipo: Journal article Palavras-chave: Avicelase; Carboxymethylcellulase; Corn steep liquor; Sugarcane bagasse.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000200008
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Extracellular production of avicelase by the thermophilic soil bacterium Bacillus sp. SMIA-2 Biological Sciences
Oliveira, Luciana Ribeiro Coutinho; Universidade Estadual do Norte Fluminense; Barbosa, João Batista; Universidade Estadual do Norte Fluminense; Martins, Meire Lelis Leal; Universidade Estadual do Norte Fluminense; Martins, Marco Antonio; Universidade Estadual do Norte Fluminense.
Nowadays, the isolation of new bacterial strains that produce enzymes with novel properties is a subject of great relevance to the scientific community. This study, in order to search for producers of new cellulase strains, investigated the avicelase production by thermophilic Bacillus sp. strain SMIA-2. The best avicelase activity was observed in a culture medium containing 0.5% (w v-1) avicel and 0.5% (w v-1) corn steep liquor with initial pH 7.5-8.0 incubated at 50oC. When avicel was replaced in the medium by the treated sugarcane bagasse (0.5%, w v-1) the avicelase activity levels were not affected. Studies on the avicelase characterization revealed that the optimum pH of the enzyme was found to be 8.5 and the enzyme retained more than 80% of its...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.07.03.00-9 Avicelase; Corn steep liquor; Sugarcane bagasse; Bacillus sp Tecnologia de Alimentos.
Ano: 2014 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/17827
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Production of Propionic Acid by Propionibacterium acidipropionici from Agroindustrial Effluents BABT
Teles,Jéssica Cristine; Stolle,Elaine Macedo; Koloda,Stela Aparecida; Barana,Ana Claudia.
Abstract The purpose of this paper was to evaluate the production of propionic acid from the fermentation of agroindustrial effluents using a Propionibacterium acidipropionici culture. The composition of the substrates was determined by using an experimental design of mixtures, resulting in 10 trials. The substrates were fermented in batch borosilicate glass reactors at a temperature of 35°C, initial pH of 6.5, and 20 mL.L-1 of inoculum suspension. The highest yield of propionic acid production, 0.79 g of product per g of substrate, was obtained with a substrate composed only of corn steep liquor, which showed a productivity of 5.20 mg.L-1h-1 and production of 0.40mL.L-1. These results showed that the corn steep liquor positively influenced performance and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agroindustrial by-products; Fermentation; Corn steep liquor; Cheese whey; Wastewater.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100702
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Brewer's spent grain and corn steep liquor as alternative culture medium substrates for proteinase production by Streptomyces malaysiensis AMT-3 BJM
Nascimento,Rodrigo Pires do; Alves Junior,Nelson; Coelho,Rosalie Reed Rodrigues.
Brewer's spent grain and corn steep liquor or yeast extract were used as the sole organic forms for proteinase production by Streptomyces malaysiensis in submerged fermentation. The influence of the C and N concentrations, as well as the incubation periods, were assessed. Eight proteolytic bands were detected through gelatin-gel-electrophoresis in the various extracts obtained from the different media and after different incubation periods, with apparent molecular masses of 20, 35, 43, 50, 70, 100, 116 and 212 kDa. The results obtained suggest an opportunity for exploring this alternative strategy for proteinases production by actinomycetes, using BSG and CSL as economically feasible substrates.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptomyces malaysiensis; Brewer's spent grains; Corn steep liquor; Proteinases; Submerged fermentation.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400020
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Media components and amino acid supplements influencing the production of fruity aroma by Geotrichum candidum BJM
Pinotti,T.; Carvalho,P.M.B.; Garcia,K.M.G.; Silva,T.R.; Hagler,Allen Norton; Leite,S.G.F..
The ability of Geotrichum candidum to produce fruity aroma in food grade sucrose, molasses, corn steep liquor and peptone based culture media was tested by sensory evaluation and analyzed by gas chromatography mass spectrometry. A strong and sweet fruity aroma was produced from molasses, with peptone or corn steep liquor stimulating aroma production. Molasses with peptone supplemented with leucine, valine, or alanine yielded better fruity aroma production and the presence of many esters was consistent with the fruity aroma production.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Geotrichum candidum; Aroma; Corn steep liquor; Molasses; Amino acids.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400017
Registros recuperados: 5
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