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Effect of epithelial debridement on human cornea proteoglycans BJMBR
Soriano,E.S.; Campos,M.S.Q.; Aguiar,J.A.K.; Michelacci,Y.M..
Corneal transparency is attributed to the regular spacing and diameter of collagen fibrils, and proteoglycans may play a role in fibrillogenesis and matrix assembly. Corneal scar tissue is opaque and this opacity is explained by decreased ultrastructural order that may be related to proteoglycan composition. Thus, the objectives of the present study were to characterize the proteoglycans synthesized by human corneal explants and to investigate the effect of mechanical epithelial debridement. Human corneas unsuitable for transplants were immersed in F-12 culture medium and maintained under tissue culture conditions. The proteoglycans synthesized in 24 h were labeled metabolically by the addition of 35S-sulfate to the medium. These compounds were extracted...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Corneal explants; De-epithelization; Glycosaminoglycan; Proteoglycan.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300005
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