|
|
|
|
|
| Coussot, Gaelle; Le Postollec, Aurelie; Incerti, Sebastien; Baque, Mickael; Faye, Clement; Vandenabeele Trambouze, Odile; Cottin, Herve; Ravelet, Corinne; Peyrin, Eric; Fiore, Emmanuelle; Vigier, Flavie; Caron, Jerome; Chaput, Didier; Przybyla, Bartos; Berger, Thomas; Dobrijevic, Michel. |
| Some microarray-based instruments that use bioaffinity receptors such as antibodies or aptamers are under development to detect signatures of past or present life on planetary bodies. Studying the resistance of such instruments against space constraints and cosmic rays in particular is a prerequisite. We used several ground-based facilities to study the resistance of aptamers to various types of particles (protons, electrons, neutrons, and carbon ions) at different energies and fluences. We also tested the resistance of aptamers during the EXPOSE-R2 mission outside the International Space Station (ISS). The accumulated dose measured after the 588 days of this mission (220 mGy) corresponds to the accumulated dose that can be expected during a mission to... |
| Tipo: Text |
Palavras-chave: Astrobiology; Cosmic rays; Biochip; Aptamers. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00601/71306/69723.pdf |
| |
|
|
| Coussot, Gaelle; Le Postollec, Aurelie; Faye, Clement; Baque, Mickael; Vandenabeele-trambouze, Odile; Incerti, Sebastien; Vigier, Flavie; Chaput, Didier; Cottin, Herve; Przybyla, Bartos; Berger, Thomas; Dobrijevic, Michel. |
| Antibody-based analytical instruments are under development to detect signatures of life on planetary bodies. Antibodies are molecular recognition reagents able to detect their target at sub-nanomolar concentrations, with high affinity and specificity. Studying antibody binding performances under space conditions is mandatory to convince space agencies of the adequacy of this promising tool for planetary exploration. To complement previous ground-based experiments on antibody resistance to simulated irradiation, we evaluate in this paper the effects of antibody exposure to real space conditions during the EXPOSE-R2 mission outside the International Space Station. The absorbed dose of ionizing radiation recorded during the 588 days of this mission (220 mGy)... |
| Tipo: Text |
Palavras-chave: Astrobiology; Cosmic rays; Biochip; Antibody; Planetary exploration. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00601/71305/69721.pdf |
| |
|
|
| Baque, M.; Dobrijevic, M.; Le Postollec, A.; Moreau, T.; Faye, C.; Vigier, F.; Incerti, S.; Coussot, G.; Caron, J.; Vandenabeele Trambouze, Odile. |
| Several instruments based on immunoassay techniques have been proposed for life-detection experiments in the framework of planetary exploration but few experiments have been conducted so far to test the resistance of antibodies against cosmic ray particles. We present several irradiation experiments carried out on both grafted and free antibodies for different types of incident particles (protons, neutrons, electrons and C-12) at different energies (between 9 MeV and 50 MeV) and different fluences. No loss of antibodies activity was detected for the whole set of experiments except when considering protons with energy between 20 and 30 MeV (on free and grafted antibodies) and fluences much greater than expected for a typical planetary mission to Mars for... |
| Tipo: Text |
Palavras-chave: Astrobiology; Biochip; Cosmic rays; Search for extraterrestrial life. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00366/47678/47809.pdf |
| |
|
|
|
