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| Mortara,Renato A.; Andreoli,Walter K.; Taniwaki,Noemi N.; Fernandes,Adriana B.; Silva,Claudio V. da; Fernandes,Maria Cecília D.C.; L'abbate,Carolina; Silva,Solange da. |
| Trypanosoma cruzi, the etiological agent of Chagas disease, occurs as different strains or isolates that may be grouped in two major phylogenetic lineages: T. cruzi I, associated with the sylvatic cycle and T. cruzi II, linked to the human disease. In the mammalian host the parasite has to invade cells and many studies implicated the flagellated trypomastigotes in this process. Several parasite surface components and some of host cell receptors with which they interact have been identified. Our work focused on how amastigotes, usually found growing in the cytoplasm, can invade mammalian cells with infectivities comparable to that of trypomastigotes. We found differences in cellular responses induced by amastigotes and trypomastigotes regarding... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Trypanosoma cruzi; Cellular invasion; Amastigotes; Trypomastigotes; Parasitophorous vacuole escape; Trafficking; Coxiella burnetii; Phylogenetic lineages. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652005000100006 |
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| Mares-Guia,Maria Angélica M.M.; Guterres,Alexandro; Rozental,Tatiana; Ferreira,Michelle dos Santos; Lemos,Elba R.S.. |
| ABSTRACT Q fever is a worldwide zoonosis caused by Coxiella burnetii—a small obligate intracellular Gram-negative bacterium found in a variety of animals. It is transmitted to humans by inhalation of contaminated aerosols from urine, feces, milk, amniotic fluid, placenta, abortion products, wool, and rarely by ingestion of raw milk from infected animals. Nested PCR can improve the sensitivity and specificity of testing while offering a suitable amplicon size for sequencing. Serial dilutions were performed tenfold to test the limit of detection, and the result was 10× detection of C. burnetti DNA with internal nested PCR primers relative to trans-PCR. Different biological samples were tested and identified only in nested PCR. This demonstrates the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Q fever; Coxiella burnetii; Molecular diagnosis; Nested PCR; IS1111. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100138 |
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