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	Provedor de dados BJM (1) Scientia Agricola (1) PAB (1) Autor Müller,Gerd Walter (2) Nunes,William Mário de Carvalho (2) Zanutto,Carlos Alexandre (2) Abreu,Patricia Antonia Estima (1) Corazza,Maria Júlia (1) Costa,Alessandra Tenório (1) Dib,Cristina Corsi (1) Gonçales,Amane Paldês (1) Miraglia,Fabiana (1) Morais,Zenaide Maria de (1) Souza,Gisele Oliveira de (1) Vasconcellos,Silvio Arruda (1) Mais... Palavra-chave Cross-protection (3) Bacterin (1) Citrus (1) Citrus sinensis (1) Hamsters (1) Pomona (1) Preimmunization (1) RFLP (1) SSCP (1) Single strand conformational polymorphism (1) Stem pitting symptoms (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2014 (1) 2013 (1) 2010 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Stability of Citrus tristeza virus protective isolates in field conditions PAB Costa,Alessandra Tenório; Nunes,William Mário de Carvalho; Zanutto,Carlos Alexandre; Müller,Gerd Walter. The objective of this work was to monitor the maintenance of Citrus tristeza virus (CTV) protective isolates stability in selected clones of 'Pêra' sweet orange (Citrus sinensis), preimmunized or naturally infected by the virus, after successive clonal propagations. The work was carried out in field conditions in the north of Paraná State, Brazil. Coat protein gene (CPG) analysis of 33 isolates collected from 16 clones of 'Pêra' sweet orange was performed using single strand conformational polymorphism (SSCP). Initially, the isolates were characterized by symptoms of stem pitting observed in clones. Then viral genome was extracted and used as template for the amplification of CPG by reverse transcription polimerase chain reaction (RTPCR). RTPCR products... Tipo: Info:eu-repo/semantics/article Palavras-chave: Citrus sinensis; Cross-protection; Preimmunization; Single strand conformational polymorphism; Stem pitting symptoms. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010000700009 Evaluation of the protective capacity of new mild Citrus tristeza virus (CTV) isolates selected for a preimmunization program Scientia Agricola Zanutto,Carlos Alexandre; Corazza,Maria Júlia; Nunes,William Mário de Carvalho; Müller,Gerd Walter. The use of tolerant rootstocks and preimmunization has satisfactorily controlled losses associated with the Citrus tristeza virus (CTV). Several researchers have shown that CTV mild isolates that are selected in the same region where they are used are superior to isolates obtained from other areas. Thus, budwoods of 20 outstanding citrus trees were collected in north and northwestern Paraná state (Brazil) citrus-producing areas and established to be used in a preimmunization program. These budwoods were tested to evaluate the potential protection of the inherently present viral complex. Based on biological indexing and molecular characterization of the capsid protein gene by RFLP (restriction fragment length polymorphism, which indicated that the plants... Tipo: Info:eu-repo/semantics/article Palavras-chave: SSCP; RFLP; Cross-protection; Citrus. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162013000200009 Cross-protection between experimental anti-leptospirosis bacterins BJM Dib,Cristina Corsi; Gonçales,Amane Paldês; Morais,Zenaide Maria de; Souza,Gisele Oliveira de; Miraglia,Fabiana; Abreu,Patricia Antonia Estima; Vasconcellos,Silvio Arruda. We investigated the existence of cross-protection between two anti-leptospirosis monovalent experimental bacterins produced with two strains of Leptospira serogroup Pomona: Fromm strain of serovar Kennewicky, isolated from pigs in the United States, and strain GR6 of serovar Pomona isolated from pigs in Brazil. Both were added of aluminum hydroxide as an adjuvant. Experimental bacterins were tested with the hamster potency test in order to assess protection provided against the disease and against the establishment of kidney infection. Controls were polyvalent commercial vaccine produced with Leptospira strains isolated outside Brazil, which included a representative of Pomona serovar, or Sorensen solution added of aluminum hydroxide adjuvant. The... Tipo: Info:eu-repo/semantics/article Palavras-chave: Pomona; Hamsters; Bacterin; Cross-protection. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300042 Registros recuperados: 3 Primeira ... 1 ... Última

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