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Mancini,Dalva Assunção Portari; Mendonça,Rita Maria Zucatelli; Pinto,José Ricardo; Mori,Enio; Fernandes,Wilson Roberto. |
This study aimed to investigate the incidence of the influenza virus, and its interspecies transmission cycle among horses. A comparative serological survey was performed using horse sera following challenge with both specific (equine) and non-specific (human) influenza virus strains. Bleedings of the 22 horses were performed during the years 1999 and 2000. Following treatment with kaolin (20%), added in rooster erythrocytes suspension (50%), for removal of non-specific antibodies, sera were titered against both Human and Equine Influenza virus, by the Hemagglutination Inhibition Assay(HI), recommended by WHO. The HI results of horse serological responses demonstrate cross reaction between both the specific strain, A/Eq1 (H7N7) ( 62.75%) and A/Eq2 (H3N8)... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Equine influenza; Human influenza; Hemagglutination Inhibition test; Cross-reaction; Protection response. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962004000600004 |
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Rodríguez,JP; De Marzi,MC; Maruñak,S; Teibler,P; Acosta,O; Malchiodi,EL; Leiva,LC. |
We examined the ability of IgG anti-crotalic PLA2 to cross-react with Bothrops spp. venoms, from snakes found in the northeast of Argentina. Immunoblotting and ELISA tests showed that IgG anti-crotalic PLA2 recognize antigens of bothropic venoms. Indirect hemolytic activity tests showed that the quantity of antibodies that neutralized 50% of Crotalus durissus terrificus venom (ED50: 2.1 mg IgG anti-crotalic PLA2/100 µg of venom) were also able to neutralize venom from other snakes in the following proportion: 34% of B. alternatus, 18% of B. diporus and 12% of B. jararacussu. Likewise, direct PLA2 activity neutralization tests showed a similar cross-neutralization pattern including 56% of B. alternatus, 29% of B. diporus and 30% of B. jararacussu. In... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bothrops; Crotalus; PLA2; Neutralization; Cross-reaction. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000300008 |
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