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Assessment of viability of sperm cells of Litopenaeus vannamei on cryopreservation BABT
Uberti,Marcela Fornari; Vieira,Felipe do Nascimento; Salência,Helena Ragibo; Vieira,Genyess da Silva; Vinatea,Luis Alejandro.
Aiming at assessing the cryopreservation potential of Litopenaeus vannamei sperm cells, 74 spermatophores were manually extracted from the sexually mature individuals. After the toxicity test to define the cryoprotectant concentration, suspensions of spermatic cells were cryopreserved in the groups in freezing solutions comprising different cryoprotectants such as dimethyl sulfoxide (DMSO) and ethylene glycol (EG) at 10% concentration. Each treatment was divided in subgroups for storage in liquid nitrogen during 0, 30, 60 and 90 days, in triplicate. After thawing at 25ºC for 40 seconds, cell viability in the suspensions was analyzed under the microscope in eosin-nigrosin stain and flow cytometry. There were no significant differences between the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cryogenics; Semen; Marine shrimp; Cell viability.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300010
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General principIes of cryosurgery in the treatment of tumors in small animals: literature review MV&Z
de Queiroz, Genilson Fernandes; Matera, Julia Maria.
Objective: To disclose the technique, equipment, mechanism of action, indications, advantages and disadvantages, complication and/or after-effects of cryosurgery in treatment of lesions. Data Sources: Data search was carried out in data base systems of CAB and MEDLINE over a 30-year term. Data Synthesis: Cryosurgery consists of the treatment of lesions through freezing (liquid nitrogen at -196° C).It has been used in medicine for the treatment of skin, liver, kidneys, prostate and oral cavity neoplasias. In. veterinary medicine it is still not broadly known and literature lacks information as to its application. It is an easily performed procedure, requiring however knowledge of the technique, of indications and possible complications and after-effects....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cryogenic surgery; Cryotherapy; Freezing techniques; Hyperthermia induced; Cryogenics; Nitrogen; Neoplasms; Pets Criocirugía. Crioterapia. Congelamiento; Hipotermia inducida; Criogénicos; Nitrógeno; Neoplasmas; Animales domésticos criocirurgia; Crioterapia; Congelamento; Hipotermia induzida; Criogênicos; Nitrogênio; Neoplasias; Animais domésticos.
Ano: 2003 URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3258
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